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第一个分子检测Borrelia sp. 在塔皮尔 (Tapirus terrestris) 中

Anna Claudia Baumel Mongruel¹, Emília Patrícia Medici^2,3,4, Ariel da Costa Canena²

¹Vector-Borne Bioagents Laboratory (VBBL), Faculdade de Ciências Agrárias e Veterinárias (FCAV), Universidade Estadual Paulista "Júlio de Mesquita Filho" (UNESP) - Jaboticabal, São Paulo, Brazil.

Veterinary research communications

|May 7, 2024

概括

细菌Borrelia theileri首次在野生南美鱼中被检测到. 这一发现扩大了这种传播病原体的已知宿主范围.

科学领域:

兽医微生物学兽医微生物学
寄生虫学的寄生虫学
野生动物健康野生动物健康

背景情况:

波雷利亚theileri是一种传染于的螺旋体,已知会影响牛,引起发烧和食欲下降.
分子诊断揭示了Borrelia theileri的新宿主和地理分布.
野生动物水库中Borrelia theileri的存在在很大程度上仍未被探索.

研究的目的:

报告在南美洲野生白 (Tapirus terrestris) 中首次分子检测到Borrelia theileri.
为了描述Borrelia sp.的遗传关系. 在塔皮尔中检测到.

主要方法:

使用针对16S rRNA基因的qPCR对99只来自巴西潘坦纳尔和塞拉多生物群的野生塔皮尔的血液DNA进行查.
阳性样本通过PCR进一步分析16SrRNA和flaB基因片段.
测序的安普利康的遗传学分析.

主要成果:

来自潘坦纳尔生物群的两只 (2.0%) 检测出Borrelia sp.的阳性. 通过qPCR.
一个阳性样本产生了可测序的amplicons用于遗传学分析.
遗传学分析表明,这是一种来自塔皮尔的Borrelia sp. 与已知的Borrelia theileri菌株组合在一起.

结论:

这项研究提供了第一个关于野生鱼中Borrelia theileri的分子证据.
这些发现扩大了已知的宿主对Borrelia theileri的敏感性.
需要进一步的研究来确定Borrelia theileri对鱼健康的影响.

关键词:

玻利利症是什么? 玻利利症是什么这是一个PCRPCR.南美南美是南美.螺旋状虫 (spirochetes) 是一种有螺旋状虫的生物.野生动物野生动物

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