精子功能的不同调节由猪卵管外细胞囊从雌性周期的不同阶段的不同调节
相关实验视频
Updated: Jun 27, 2025
09:30
Phosphopeptide Analysis of Rodent Epididymal Spermatozoa
Published on: December 30, 2014
12.9K
S M Toledo-Guardiola1, P Martínez-Díaz1, R Martínez-Núñez1
1Department of Physiology, Faculty of Veterinary Science, International Excellence Campus for Higher Education and Research 'Campus Mare Nostrum', University of Murcia, Murcia, Spain.
Reproduction, fertility, and development
|May 7, 2024
概括
猪卵管外细胞囊 (oEVs) 调节精子功能在雌性周期中不同. 晚期的卵泡oEV增强了精子结合,但降低了生育能力,而质oEV降低了精子的运动性.
科学领域:
- 生殖生物学 生殖生物学
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 卵管液中的细胞外囊泡 (EVs) 对于哺乳动物的繁殖至关重要.
- oEVs影响精子容量,受精和早期胚胎发育.
研究的目的:
- 在整个雌性周期 (卵泡晚期,白色早期,白色中期,白色晚期) 中对猪oEV (poEV) 进行表征.
- 调查这些poEVs对野猪精子功能和体外生育能力的影响.
主要方法:
- 对poEVs的隔离和表征.
- 对poEV与精子结合的评估.
- 对精子运动,活力,体状况和酸化 (pPKA,Tyr-P) 的分析.
- 在体外受精参数的评估.
主要成果:
- 鉴定出poEVs是囊泡,其尺寸,数量或蛋白质度在雌性周期阶段之间没有显著差异.
- 晚期卵泡 (LF) poEVs 显示出更高的精子结合亲和力.
- 早期色期 (EL),中期色期 (ML) 和晚期色期 (LL) 的poEVs降低了精子运动性.
- LF,EL和ML poEVs降低了蛋白激酶A酸化 (pPKA) 的水平.
- LF poEVs降低了精子透率和每个透的卵细胞中的精子.
结论:
- 来自不同雌性周期阶段的poEV通过与精子细胞的相互作用来差异调节精子功能.
- poEVs影响精子运动,电容和精子-卵细胞相互作用.
- LF和LL poEVs的独特效果表明在试管婴儿系统中有潜在的应用,以调节生殖过程.
相关概念视频
01:15Sperm Transport
778
The journey of sperm from its origin to the point of ejaculation begins within the seminiferous tubules of the testis. Here, Sertoli cells produce fluid that propels non-motile sperm through a series of conduits, starting with the straight tubules leading to the rete testis. This interconnected network of tubules acts as the initial pathway for sperm, guiding them into the efferent ductules and then into the epididymis for maturation.
The maturation phase occurs in the epididymis, where sperm...
The maturation phase occurs in the epididymis, where sperm...
778
01:27Ovarian Cycle
1.2K
The menstrual cycle includes a critical component known as the ovarian cycle, which undergoes two main phases each month—the follicular phase and the luteal phase. The follicular phase is variable and averaging around 14 days. Ovulation, triggered by a surge in luteinizing hormone (LH), marks the transition between the two phases. The second phase, the luteal phase, is relatively consistent, lasting approximately 14 days, and is marked by the activity of the corpus luteum. While a cycle...
1.2K