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多组织转录组分析将组织特异性circRNAs与肉料摄入量和效率的异构之间的关联联系起来

Jingwei Yuan¹, Qin Li¹, Yanyan Sun¹

¹Key Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

Poultry science

|May 7, 2024

概括

循环RNAs (circRNAs) 在异质中起着重要的作用,提高料效率. 这项研究揭示了与剩余料摄入 (RFI) 相关的组织特异性circRNAs,并确定了预测RFI的潜在生物标志物.

科学领域:

动物遗传学动物遗传学
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

在养中,异构或混合活力至关重要,这是由非添加基因遗传驱动的.
循环RNAs (circRNAs) 在异质化中的特定作用,特别是对于料效率特征,如残留料摄入量 (RFI),仍然在很大程度上未被探索.

研究的目的:

为了研究circRNAs在中RFI异构中的参与.
用多组织转录组分析阐明料摄入和效率中circRNAs的调节机制.

主要方法:

在杂交的下丘脑,肝脏,十二指肠和卵巢中概述circRNA景观.
使用权重基因共同表达网络分析 (WGCNA) 来识别与RFI相关的circRNA-mRNA共同表达模块.
使用RT-qPCR验证关键的circRNA基因相关性.

主要成果:

非添加式表达是杂交中circRNA的主要遗传模式,具有普遍的组织特异性表达.
与RFI相关的CircRNA-mRNA模块在下丘脑和肝脏中被确定,富含氧化酸化通路.
八个非添加性circRNA被发现与16个非添加性基因高度相关,对RFI产生负面影响,Circ-ITSN2在肝脏组织中得到验证.

结论:

这项研究提供了第一个多组织转录的证据,将组织特异的circRNA与的料效率的异质化联系起来.
鉴定到的circRNA为异构的分子基础提供了新的见解,并可以作为预测RFI及其相关异构的潜在生物标志物.

关键词:

肉肉肉肉循环RNA 是一个循环RNA.料效率料效率是指料的使用效率.异质化是一种异质化.

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