与绳子面包相关的有氧子形成细菌:识别,表征和破坏潜力的评估
Nicola Pacher1, Johanna Burtscher1, Denisse Bender1
1University of Natural Resources and Life Sciences, Vienna, Department of Food Science and Technology, Institute of Food Science, Muthgasse 18, 1190 Vienna, Austria.
International journal of food microbiology
|May 7, 2024
概括
这项研究引入了一种标准化的方法,用于评估空气性子形成 (ASF) 细菌在面包腐烂中的绳索诱导潜力 (RIP). 鉴定出新的细菌物种,客观色度测量量损坏的数量,有助于食品质量控制.
科学领域:
- 食品微生物学 食品微生物学
- 细菌破坏的破坏
- 食品质量评估 食品质量评估
背景情况:
- 众所周知,有氧子形成 (ASF) 细菌会导致面包的状,导致经济损失和潜在的健康风险.
- 现有的评估面包腐烂的方法往往是主观的,缺乏对物理化学特征的系统量化.
- 毒性ASF细菌菌株很常见,因此需要可靠的方法来评估它们的破坏潜力.
研究的目的:
- 为了识别和描述与绳子面包腐烂相关的ASF细菌.
- 开发和验证一种标准化,客观的方法来评估细菌菌株的绳索诱导潜力 (RIP).
- 调查与腐烂相关的ASF细菌中的潜在毒性因素.
主要方法:
- 使用MALDI-TOF-MS识别82种ASF细菌分离物,其物种身份通过16SrRNA和gyrA或panC基因测序得到确认.
- 使用色度测量测量标准化协议的开发,以客观地评估RIP,通过用细菌子注射自封式面包片.
- 对毒性因素的研究,包括蜂群运动性和血液溶解.
主要成果:
- 确定了Bacillus velezensis,Bacillus inaquosorum和Bacillus spizizenii作为可能导致绳面包腐烂的物种.
- 建立了用于RIP评估的标准化分类协议,利用色度测量来客观量化面包变色.
- 观察到诱导绳子腐烂的菌株经常表现出蜂群运动性和血液溶解等毒性因素.
结论:
- 这项研究提供了一个强大的,客观的方法来评估ASF细菌在面包中的破坏潜力.
- 这些发现扩大了涉及面包腐烂的细菌物种清单,并突出了毒性因素的重要性.
- 开发的协议为食品质量控制和行业的安全提供了重大进展.
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