使用半固态发酵和人工神经网络优化Aureobasidium pullulans的pullulan生产:与Ag-TiO2纳米复合材料浸的pullulan的特征和抗菌活性
Noha M Eldadamony1, Abeer A Ghoniem2, Abdulaziz A Al-Askar3
1Seed Pathology Department, Plant Pathology Research Institute, Agricultural Research Center, Giza 12619, Egypt.
概括
人工神经网络通过在法巴豆生物质上的半固态发酵优化了普鲁兰的生产,实现了创纪录的产量. 纯化的Pullulan表现出独特的特性,并增强纳米粒子抗菌活性.
科学领域:
- 生物技术是生物技术.
- 生物材料科学 生物材料科学
- 发酵技术的发酵技术
背景情况:
- 普鲁兰是一种多用途的多糖,在食品,医药和工业中都有应用.
- 当前的生产方法往往在产量和成本效益方面面临限制.
- 法巴豆生物质为微生物发酵提供了一个未充分利用的,可持续的基质.
研究的目的:
- 使用人工神经网络 (ANN) 在法巴豆生物质 (FBB) 上进行半固态发酵 (S-SSF).
- 为了描述产生的pullulan的物理化学特性和抗氧化活性.
- 研究pullulan在增强Ag@TiO2纳米颗粒的抗菌功效方面的协同作用.
主要方法:
- 人工神经网络 (ANN) 用于优化S-SSF参数以获得普卢兰产量.
- 普鲁兰被净化并使用包括热分析,光谱 (EDS) 和显微镜 (SEM) 在内的技术进行了表征.
- 在与Fusarium oxysporum合成期间,Pullulan与Ag@TiO2纳米颗粒集成.
主要成果:
- 在10.82天内实现了创纪录的pullulan产量36.81 mg/g,明显超过了之前的基准.
- 鉴定结果显示,pullulan具有高热稳定性,无形结构和显著的抗氧化活性.
- 与Ag@TiO2纳米颗粒的集成导致了增强的纳米颗粒稳定性,并显著改善了对人类病原体的抗菌活性.
结论:
- 在FBB上对ANN优化的S-SSF为高产普鲁兰生产提供了可持续和成本效益的途径.
- 普鲁兰的独特性质使其在本质上和作为纳米材料的性能增强剂具有价值.
- 这项研究表明了开发具有广泛应用潜力的先进抗微生物纳米材料的新方法.
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