来自巴西人工水源的莱吉欧内拉肺炎菌分离物的遗传多样性
Dândrea Driely de Melo Ferrari1, Solange Costa Lima2, Raquel Lima Figueiredo Teixeira3
1Laboratório de Biologia Molecular Aplicada a Micobactérias, Instituto Oswaldo Cruz (Fiocruz), Rio de Janeiro, RJ, 21040-360, Brazil. dan.mferrari@hotmail.com.
Current microbiology
|May 7, 2024
概括
在巴西对军团菌的基因分析揭示了包括新型在内的多种菌株,突出了对军团队员的公共卫生监测和控制措施的迫切需要.
科学领域:
- 环境微生物学环境微生物学
- 细菌遗传学 细菌遗传学
- 公共卫生 公共卫生
背景情况:
- 军团菌肺炎 (Lp) 在吸入受污染的气溶后引起严重的肺炎 (军团病).
- 有限的数据存在于莱吉奥内拉菌 spp. 在巴西,需要进行本地菌株表征.
研究的目的:
- 为了研究Legionella spp.的遗传多样性. 从巴西的各种环境中分离出来.
- 在巴西识别出已知的和新的军团菌的序列类型 (ST).
主要方法:
- 收集了40种军团菌种. 来自巴西9个州的不同来源的隔离物.
- 使用了基于序列的排列 (SBT),涉及七个关键基因 (flaA,pilE,asd,mip,mompS,proA,neuA) 的测序.
- 定义的序列类型 (STs) 用于分子识别和比较.
主要成果:
- 在34/40个分离物中确定了11个序列类型 (ST).
- 发现了三个新型ST (ST2960,ST2962,ST2963),其中一个在圣保罗冷却塔中发现.
- ST1是最常见的,与全球分布一致,其他已识别的ST与世界各地的人类军团病毒感染有关.
结论:
- 巴西雷吉昂氏菌分离物表现出显著的遗传多样性,包括新型菌株.
- 已知致病性STs的存在强调了巴西军团病的潜在风险.
- 建议在巴西实施强有力的监测系统和增强的基因型识别方法,以控制军团菌.
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