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Updated: Jun 27, 2025

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tauFisher从单个批量和单细胞伪批量转录数据的单个样本中预测昼夜时间

Junyan Duan^1,2, Michelle N Ngo^1,2, Satya Swaroop Karri³

¹Center for Complex Biological Systems, University of California Irvine, Irvine, CA, USA.

Nature communications

|May 7, 2024

概括

这项研究介绍了tauFisher,这是一个计算工具,可以从转录组数据中预测昼夜时间. 这种方法通过精确的时间标记生物样本,有助于昼夜医学和研究.

科学领域:

时间生物学时间生物学
计算生物学计算生物学
基因组学就是基因组学.

背景情况:

循环时钟调节了重要的生物过程.
扰乱昼夜节律与各种疾病有关.
准确确定昼夜时间对于昼夜医学和研究至关重要.

研究的目的:

为了介绍tauFisher,一个用于预测昼夜时间的新型计算管道.
为了验证tauFisher在各种转录样本和实验条件中的准确性.

主要方法:

tauFisher在单个转录组样本中分析了节律基因之间的相关性.
该管道在批量和单细胞RNA测序数据上进行了测试.
应用于小鼠皮肤单细胞RNAseq数据,以评估细胞类型特定的节奏.

主要成果:

tauFisher从转录基因样本准确地预测了昼夜时间.
在多种组织类型和实验环境中表现出强度.
与纤维细胞相比,皮肤免疫细胞中揭示了潜在的昼夜阶段异质性.

结论:

tauFisher是用于昼夜时间预测的多功能和准确工具.
该管道适用于各种数据类型,包括单细胞RNA测序.
促进昼夜医学和生物研究的进步.

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Circadian Rhythms and Gene Regulation

Circadian Rhythms and Gene Regulation

The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent...