在红色素形成过程中,miR-144/Hmgn2调节轴调节色素组织
Dmitry A Kretov1, Leighton Folkes2, Alexandra Mora-Martin1
1Department of Biochemistry and Cell Biology, Boston University Chobanian & Avedisian School of Medicine, Boston, MA, USA.
Nature communications
|May 7, 2024
概括
微RNA-144 (miR-144) 和它的目标基因Hmgn2对于红细胞发育至关重要. 这项研究揭示了它们在脊椎动物中红细胞成熟期间调节染色质凝聚中的作用.
科学领域:
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
- 细胞生物学 细胞生物学
背景情况:
- 干细胞和祖细胞的分化是一个复杂的,受规范的过程.
- 转录后调节在细胞分化中起着关键作用.
- 染色体调节对于细胞成熟至关重要.
研究的目的:
- 调查微RNA-144 (miR-144) 在红色素生成过程中的染色素调节中的作用.
- 识别和描述与染色体组织有关的miR-144的点.
- 阐明miR-144的调节轴及其在红细胞末端分化中的目标.
主要方法:
- 使用斑马鱼和人类诱导的多能干细胞 (iPSC) 衍生红色素细胞.
- 采用基因操纵来研究miR-144/Hmgn2调节轴.
- 分析了染色体凝聚和红细胞成熟的表型.
主要成果:
- 在红细胞成熟过程中,miR-144的损失会损害染色质凝结.
- miR-144调节Hmgn2的表达,这是染色体组织的关键因素.
- 在Hmgn2 3'UTR中完整的miR-144点对于适当的红细胞成熟至关重要.
- 失去Hmgn2部分挽救了在miR-144缺乏细胞中观察到的表型.
结论:
- miR-144及其点Hmgn2形成了脊椎动物红细胞末端分化的关键调节电路.
- 这个轴调节染色体凝聚,这是红细胞成熟的关键事件.
- 这些发现强调了转录后调节在控制发育过程中的重要性.
更多相关视频
06:40Mouse Fetal Liver Culture System to Dissect Target Gene Functions at the Early and Late Stages of Terminal Erythropoiesis
Published on: September 9, 2014
15.1K
10:28Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
Published on: September 20, 2018
6.4K
相关概念视频
02:23Master Transcription Regulators
6.9K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.9K
02:41Chromatin Structure Regulates pre-mRNA Processing
7.0K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
The chromatin structure, especially...
7.0K
02:25Spreading of Chromatin Modifications
8.3K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
Writers
The writer...
8.3K
02:35Chromatin Position Affects Gene Expression
23.3K
Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area.
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
23.3K
02:36RNA Polymerase II Accessory Proteins
9.2K
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
9.2K
01:30General Transcription Factors
5.3K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
5.3K