猪的cis-acting lnc-CAST通过素H3K27acac积极调节CXCL8的表达
Junxin Gao1,2, Haidong Yu2, Yu Pan2
1College of Veterinary Medicine, Southwest University, Chongqing, 400715, China.
Veterinary research
|May 7, 2024
概括
一种新的长非编码RNA,Inc-CAST,调节了猪巨细胞中CXCL8 (中性粒细胞化学反应因子) 的产生. 这一发现对于了解猪生殖和呼吸系统综合征病毒 (PRRSV) 感染期间的炎症和肺损伤至关重要.
科学领域:
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
- 病毒学 病毒学
背景情况:
- 猪生殖和呼吸系统综合征病毒 (PRRSV) 通过向肺膜巨细胞 (PAMs) 引起急性肺感染.
- 像CXCL8 (中性细胞化学反应因子) 这样的化学因子在病毒感染期间的炎症反应和免疫反应中至关重要.
- 长非编码RNAs (lncRNAs) 越来越多地被认为是它们在细胞过程中的调节作用.
研究的目的:
- 在PRRSV感染的背景下,研究位于Cxcl8基因位点内的新型lncRNA,lnc-CAST的作用.
- 为了确定猪巨体中Inc-CAST和CXCL8表达之间的调节关系.
- 阐明Inc-CAST影响CXCL8产生和随后的细胞反应的潜在分子机制.
主要方法:
- 定量实时PCR (qRT-PCR) 用于评估Inc-CAST和CXCL8表达水平.
- 在猪巨中治疗PRRSV感染,脂多糖 (LPS) 和脂铁醇酸 (LTA).
- 在PAM中对inc-CAST进行过度表达和敲除实验.
- 细胞迁移对上皮细胞和中性粒细胞招募的测试.
- 染色体免疫沉 (ChIP) 试验用于分析CXCL8促进体中的组蛋白修饰.
主要成果:
- 在PRRSV感染,LPS或LTA治疗后,Inc-CAST和CXCL8在PAM和猪肺中显著上调.
- 过度表达lnc-CAST增加了CXCL8的产生,增强了上皮细胞的迁移和中性粒细胞的招募.
- 抑制lnc-CAST降低了CXCL8的产生,减少了上皮细胞迁移和中性粒细胞的招募.
- 从机制上讲, lnc-CAST,一个核局部化的 lncRNA,在CXCL8促进体上促进组分素H3K27的乙化,从而上调CXCL8转录在 cis.
结论:
- lnc-CAST是猪巨细胞中CXCL8表达的关键阳性调节剂.
- 在PRRSV感染期间,lnc-CAST在调解炎症反应,包括中性粒细胞招募方面发挥着重要作用.
- 这项研究为IncRNA介导的化学生产调节及其对病毒诱导的肺损伤的影响提供了新的见解.
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