关于Staphylococcus aureus中耐药性决定因素的表达和沉默的分子洞察力
Muhammad Ijaz1, Muhammad Jawad Sabir1, Muhammad Umar Javed1
1Department of Veterinary Medicine, University of Veterinary and Animal Sciences, Lahore, Pakistan.
概括
抗微生物药物耐药的金黄色葡萄球菌菌株,包括耐甲基金黄色葡萄球菌 (MRSA),在巴基斯坦牛奶中普遍存在. 这些与生物膜基因相关的耐药菌株突出显示了潜在的传播风险和诊断差异.
科学领域:
- 兽医微生物学 兽医微生物学
- 抗微生物耐药性 抗微生物耐药性
- 分子流行病学分子流行病学
背景情况:
- 牛的亚临床乳腺炎是巴基斯坦的一个重大问题.
- 黄金葡萄球菌 (Staphylococcus aureus) 是与牛乳腺炎相关的主要病原体.
- 了解抗菌素耐药性模式对于公共卫生和动物福利至关重要.
研究的目的:
- 调查巴基斯坦抗菌素耐药性黄金葡萄球菌的流行情况.
- 确定生物膜形成基因与抗菌素耐药性之间的关联.
- 为了确定基因型和表型抗性检测方法之间的差异.
主要方法:
- 采集了384个牛奶样本,并对亚临床乳腺炎进行了查.
- 鉴定了金黄色葡萄球菌分离体,并使用Kirby-Bauer光盘扩散方法对其进行了抗微生物敏感性测试.
- 使用聚合酶连锁反应 (PCR) 进行基因型分析,以检测特定的耐药基因 (例如,mecA,blaZ,vanB,tetK,accA-aphD) 和生物膜基因 (icaA,icaD).
主要成果:
- 亚临床乳腺炎和黄金葡萄球菌的患病率分别为59.11%和46.69%.
- 对于耐甲西林黄金色杆菌 (MRSA) (44.33%),抗β-乳酸盐黄金色杆菌 (BRSA) (58.49%) 和其他类型的菌种,表型耐药性很高.
- PCR证实了MRSA (46.80%),BRSA (37.09%) 和VRSA (36.36%) 的显著比例,其中生物膜基因 (icaA,icaD) 分别在49.06%和40.57%的分离物中检测到.
- 在基因型和表型抗性识别之间观察到很大的差异,特别是在accA-aphD基因中.
结论:
- 抗生素耐药的金黄色葡萄球菌菌株在巴基斯坦普遍存在,构成传播给人类的潜在风险.
- 多种耐药性决定因素和机制有助于抗生素耐药性基因的表达和沉默.
- 诊断方法的差异需要仔细解释耐药性概况.
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