评价Sonicate流体培养的截止点,以诊断周围假肢关节感染
Judith Alvarez Otero1, Melissa J Karau1, Kerryl E Greenwood-Quaintance1
1Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA.
Open forum infectious diseases
|May 8, 2024
概括
在超声波液培养中≥20 CFU/10mL的切断值是诊断周围假肢关节感染 (PJI) 的理想选择. 这一发现有助于标准化PJI的诊断标准,改善患者的治疗结果.
科学领域:
- 整形外科手术 整形外科手术
- 传染性疾病 传染性疾病
- 临床微生物学 临床微生物学
背景情况:
- 周围假肢关节感染 (PJI) 诊断依赖于从假肢部件中识别病原体.
- 植入物超声波是病原体恢复的关键方法,但最佳培养结果的解释有所不同.
- 需要对PJI进行标准化诊断标准,以提高准确性和患者管理.
研究的目的:
- 为了确定超声波液体培养的理想切断值,结果在诊断PJI.
- 建立可靠的定量值来识别PJI病原体.
主要方法:
- 从部和膝盖假肢组件 (2007-2020) 中分析了1448种超声波液体培养物.
- 采集的样品经过和在林格溶液中进行超声波处理,然后进行离心.
- 报告了半量化 (CFU/10mL) 的培养结果,并评估了不同截止点的灵敏度和特异性.
主要成果:
- 在1448例中,共有644例 (44%) 被诊断为PJI.
- 灵敏度从75.0% (<20 CFU/10mL) 到39.8% (>100 CFU/10mL) 不同.
- 在≥20CFU/10mL时,特异性达到99.8%,在更高的切断点时达到100%.
结论:
- 超声波液培养切断值≥20CFU/10mL为PJI诊断提供了适当的灵敏度和高特异性的平衡.
- 这一门可以帮助标准化PJI诊断协议.
- 精细化培养解释提高了诊断周围假肢关节感染的可靠性.
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