评估化作为移动相添加剂的化在电喷离子化中提高灵敏度的评估
Jeremy Ryan McFadden1, David Michael Ames1
1Crop Protection Discovery and Development Corteva Agriscience Indianapolis Indiana USA.
Analytical science advances
|May 8, 2024
概括
化可以增强电子喷射电离质谱仪对小分子的灵敏度. 它显著改善了负离子模式中的检测,并在正离子模式中显示了可变的好处.
科学领域:
- 分析化学 分析化学
- 质谱测量质量谱测量
- 分离科学 分离科学
背景情况:
- 电子喷雾电离 (ESI) 与质谱法 (MS) 结合,是一种强大的分析技术.
- 化以前已经证明了提高ESI-MS灵敏度的潜力,特别是在类固醇分析中.
研究的目的:
- 调查化作为移动相添加剂对各种小分子的检测灵敏度的影响,使用ESI-MS液态染色学.
- 在优化的仪器条件下,比较化与其他常见添加剂 (如酸和氧化) 的有效性.
主要方法:
- 使用了传统的液态色谱与电喷射电离质谱法 (LC-ESI-MS).
- 对9种不同的小分子进行了全源优化,其有机度各不相同 (30%,60%,90%).
- 评估的移动相添加剂:化,酸和氧化.
主要成果:
- 化对所有测试化合物的负ESI灵敏度持续提高了2-22倍.
- 阳性ESI显示混合结果:7个化合物中的4个化合物经历了1-11倍的灵敏度改善,而3个没有显示任何变化.
- 使用化氨的优化源条件产生了显著的灵敏度增长,特别是在负离子模式下.
结论:
- 化是一种高度有效的添加剂,可增强ESI-MS的灵敏度,特别是用于小分子负离子分析.
- 在正离子模式下化氨的实用性取决于化合物,这表明了特定模拟分析的潜力.
- 采用化的优化LC-ESI-MS方法可以大幅提高分析检测极限.
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