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在DICER1相关瘤中,在lysine 27免疫染模式上的histon H3三甲基化

Murad Alturkustani^1,2,3,4, Bo Yang^1,5, Crystal Bockoven^1,2

¹Department of Pathology and Laboratory Medicine, Children's Hospital Los Angeles, Los Angeles, CA, USA.

Translational pediatrics

|May 8, 2024

概括

在氨酸27 (H3K27me3) 损失上发生的希斯H3三甲基化在DICER1相关的瘤中并不普遍. 然而,马赛克H3K27me3损失是II型和III型肺质瘤的诊断标志物.

科学领域:

在瘤学瘤学.
遗传学是一个遗传学.
病理学病理学病理学

背景情况:

与DICER1相关的瘤在多个器官中表现出显著的异质性.
与DICER1突变相关的原发性内肉瘤通常通过免疫组织化学在细胞核中对氨酸27 (H3K27me3) 呈现素H3三甲基化损失.

研究的目的:

在各种与DICER1相关的瘤中研究H3K27me3免疫染模式.
为了确定H3K27me3损失是否是不同DICER1相关瘤中一致的标志物.

主要方法:

从11名确诊DICER1突变的患者中提取了12种瘤和4种多肺母细胞瘤 (PPB) 瘤.
使用抗H3K27me3抗体对所有检索的瘤样本进行了H3K27me3免疫染.

主要成果:

在塞尔托利-莱迪格细胞瘤的塞尔托利细胞组件中观察到H3K27me3的马赛克损失.
与DICER1相关的内肉瘤显示完全或马赛克H3K27me3损失.
肺母细胞瘤II和III类型始终表现出马赛克H3K27me3在线索细胞组件中的损失,使其与其他瘤类型区别开来.

结论:

在所有与DICER1相关的瘤中,H3K27me3表达并不普遍丢失,并且它的缺失不能预测DICER1突变状态.
H3K27me3的马赛克区域损失是II和III型肺质瘤的有价值的诊断标记,表明与DICER1相关的内肉瘤具有共同的特征.

关键词:

DICER1 的意思是说.塞尔托利-莱迪格细胞瘤在素27 (H3K27me3) 上进行素H3三甲基化.免疫组织化学免疫组织化学腹肺母细胞瘤 (PPB) 是一种

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