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在Scatophagus argus的淋巴腺发育过程中,矩阵金属蛋白酶基因家族的进化和基因表达

Renchi Zheng¹, Hongjuan Shi¹, Xiaoying Ru¹

¹Guangdong Province Famous Fish Reproduction Regulation and Breeding Engineering Technology Research Center of Engineering Technology Research Center, Fisheries College of Guangdong Ocean University Zhanjiang 524088, Guangdong, China.

American journal of translational research

|May 8, 2024

概括

这项研究在Scatophagus argus中确定了23个矩阵金属蛋白酶 (MMP) 基因,揭示了男性和女性生殖腺发育至关重要的特定MMP. 这些发现突出了MMPs在远生物进化和繁殖中的作用.

科学领域:

基因组学和分子生物学
进化生物学进化生物学
生殖生物学生殖生物学

背景情况:

斯卡托法古斯 (Scatophagus argus) 的繁殖周期涉及淋巴腺退化和重新成熟,细胞外矩阵 (ECM) 蛋白质降解.
矩阵金属蛋白酶 (MMPs) 是一种蛋白酶家族,是ECM降解的关键媒介.

研究的目的:

在Scatophagus argus中识别MMP基因家族.
为了确定MMPs在不同淋巴腺发育阶段的基因表达水平.

主要方法:

使用BLAST和HMMER对MMP基因进行全基因组识别.
为了获得进化见解,进行了遗传学和合成分析.
定量逆转录聚合酶连锁反应 (RT-qPCR) 用于分析不同发育阶段的生殖腺中MMP基因表达.

主要成果:

在S. argus基因组中确定了23个MMP基因,与其他脊椎动物相比,teleosts显示出更高的基因拷贝数.
在发生性腺发育期间,11个MMP基因在丸或卵巢上升调节.
特定的MMP基因 (例如,mmp2,9,14a) 在丸中表达更高,而mmp28在卵巢发育中更为突出. 部表达最低,但一些MMP在所有组织中表达高.

结论:

整体基因组复制是MMP基因家族在teleosts中扩大的主要驱动因素.
像mmp2,9,14a,15a,15b,16a,17a,23b和24这样的MMP基因对丸发育至关重要,而mmp28对卵巢发育至关重要.
这项研究加深了对远程生物MMP基因家族演化的理解,并为未来对S. argus繁殖中MMPs的研究提供了基础.

关键词:

MMP 基因家族的基因家族.生物信息学分析分析 gonadal MMP 的基因表达方式.

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