Circ_0072088通过调节miR-607/RNF2轴促进乳腺癌的进展
Mingxi Chen1, Yang Hui2, Bo Tian3
1Department of Surgery, Xi'an Hospital of Traditional Chinese Medicine Xi'an 710021, Shaanxi, China.
American journal of translational research
|May 8, 2024
概括
循环RNAcirc_0072088通过对miR-607进行下调和对RNF2进行上调来促进乳腺癌的进展. 这导致细胞增殖和细胞循环进展的增加,同时减少癌细胞的亡.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 基因规则 基因规则
背景情况:
- 循环非编码RNAs (circRNAs) 越来越多地被认为是它们在各种癌症中的作用.
- 在乳腺癌 (BC) 进展中circ_0072088的特定功能仍然在很大程度上未被探索.
- 了解BC发展背后的分子机制对于向治疗至关重要.
研究的目的:
- 为了研究circ_0072088在乳腺癌 (BC) 进展中的作用.
- 阐明circ_0072088影响BC细胞行为的分子机制.
- 在circ_0072088/miR-607/RNF2路径中识别潜在的治疗点.
主要方法:
- 使用定量实时PCR测量BC组织和细胞系中的circ_0072088,miR-607和RNF2mRNA水平.
- 使用CCK-8和BrdU整合试验来评估细胞增殖.
- 细胞周期分布和细胞亡通过流动细胞计分析.
主要成果:
- 与正常组织相比,BC组织中的Circ_0072088和RNF2水平升高,而miR-607水平降低.
- 过度表达circ_0072088增强了BC细胞增殖和细胞周期进展,并抑制了细胞亡.
- 沉默circ_0072088扭转了这些影响,表明其致癌作用.
- Circ_0072088直接针对miR-607,导致其目标RNF2.2的升级调节.
- 过度表达的MiR-607抵消了circ_0072088.8引起的增殖和抗亡效应.
结论:
- Circ_0072088 在乳腺癌中作为瘤基因起作用.
- 该circ_0072088/miR-607/RNF2轴在促进BC细胞增殖和生存方面发挥着至关重要的作用.
- 向circ_0072088可能代表乳腺癌的新疗法策略.
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