Hsa_circ_0051908 促进肝细胞癌的进展,通过调节上皮细胞-半细胞转变过程
Yinbing Wu1, Huafei Tang1, Shuzhong Cui1
1Department of Hepatobiliary Surgery, Affiliated Cancer Hospital and Institute of Guangzhou Medical University, Guangzhou, China.
Analytical cellular pathology (Amsterdam)
|May 8, 2024
概括
Hsa_circ_0051908通过增强细胞增殖和迁移,促进肝细胞癌 (HCC) 的进展. 沉默这种循环RNA会抑制瘤生长,并可能为HCC提供一种新的治疗策略.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 肝细胞癌 (HCC) 是一种普遍存在的恶性瘤,具有复杂的分子基础.
- 循环RNAs (circRNAs) 正在成为各种癌症的关键调节者,包括HCC.
研究的目的:
- 研究hsa_circ_0051908在肝细胞癌 (HCC) 进展中的作用和机制.
- 评估hsa_circ_0051908作为HCC的治疗点的潜力.
主要方法:
- 定量实时PCR (RT-qPCR) 用于表达式分析.
- 细胞检测 (CCK-8,EDU,TUNEL,流细胞计,transwell) 用于扩散,亡,入侵和迁移.
- 西方涂抹用于分子机制调查.
- 在体内瘤生长研究.
主要成果:
- 在HCC组织和细胞系中,Hsa_circ_0051908的表达被上调.
- 镇压hsa_circ_0051908抑制了HCC细胞的增殖,迁移和入侵,同时增加了细胞亡.
- 沉默hsa_circ_0051908受影响的上皮介质转变 (EMT) 标志物 (维门丁,Snail,E-cadherin).
- 在体内,hsa_circ_0051908的抑制显著降低了瘤的生长.
结论:
- Hsa_circ_0051908促进了HCC的进展,部分是通过调解EMT.
- Hsa_circ_0051908代表了肝细胞癌治疗的潜在治疗标.
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