相关实验视频
Updated: Jun 27, 2025

07:22
Rapid Isolation of Dorsal Root Ganglion Macrophages
Published on: September 7, 2019
10.3K
血小板激活因子产生巨/微细胞的短暂增加与神经病痛有关
Shota Yamamoto1,2, Tomomi Hashidate-Yoshida1, Yuki Yoshinari1,3
1Department of Lipid Life Science, National Center for Global Health and Medicine, Shinjuku-ku, Tokyo 162-8655, Japan.
iScience
|May 8, 2024
概括
外周神经损伤通过增加血小板激活因子 (PAF) 引起神经病痛. 巨细胞和微质细胞驱动这种增加,表明PAF信号作为疼痛治疗的目标.
科学领域:
- 神经科学是一个神经科学.
- 免疫学 免疫学 免疫学
- 生物化学 生物化学
背景情况:
- 周围神经损伤 (PNI) 会导致神经病痛和触觉性全神经损伤.
- 在PNI后观察到基因和蛋白质表达的改变,以及脂质的变化.
- 血小板激活因子 (PAF),一种炎症性脂质媒介,与慢性疼痛有关.
研究的目的:
- 研究PAF在PNI引起的神经病痛中的作用.
- 为了识别PNI后PAF的细胞源和信号通路.
- 探索PAF-PAF受体信号作为潜在的治疗点.
主要方法:
- 利用淘汰赛小鼠模型,针对巨细胞和微质细胞中的PAF产生酶 (LPCAT2).
- 给野生类型,淘汰赛和PAF受体淘汰赛小鼠的PNI.
- 测量PAF水平在背部根 (DRG) 和脊髓组织中.
- 评估了机械形作为一种疼痛行为读数.
主要成果:
- 在DRG和脊髓中,PNI暂时增加了PAF水平.
- 巨/微细胞特异性LPCAT2淘汰小鼠在PNI后没有发展机械体或显示增加的PAF.
- 在PAF受体淘汰赛小鼠中,在脊髓中表现出抑制的PNI诱导的PAF增加.
- 在PNI中观察到PAF自我放大循环的证据.
结论:
- 巨细胞和微质细胞在PNI后对PAF的产生有显著的贡献.
- 在PNI诱导的神经病痛的发展中,PAF信号发挥着至关重要的作用.
- 准PAF-PAF受体通路为神经病痛疼痛管理提供了一个有希望的策略.
更多相关视频
08:52Neuron-Macrophage Co-cultures to Activate Macrophages Secreting Molecular Factors with Neurite Outgrowth Activity
Published on: March 30, 2018
8.8K
08:43Intravital Imaging of Axonal Interactions with Microglia and Macrophages in a Mouse Dorsal Column Crush Injury
Published on: November 23, 2014
11.5K
相关概念视频
Nociception
27.9K
Nociception—the ability to feel pain—is essential for an organism’s survival and overall well-being. Noxious stimuli such as piercing pain from a sharp object, heat from an open flame, or contact with corrosive chemicals are first detected by sensory receptors, called nociceptors, located on nerve endings. Nociceptors express ion channels that convert noxious stimuli into electrical signals. When these signals reach the brain via sensory neurons, they are perceived as pain.
27.9K
Formation of the Platelet Plug
5.9K
The platelet phase, the second stage of hemostasis, commences around 15-20 seconds after an injury. It follows and overlaps with the vascular phase, during which blood vessels constrict to minimize blood loss.
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...
5.9K
Inflammation
53.4K
Overview
53.4K
Analgesia and Pain Management
584
Pain is critical to various clinical pathologies, provoking an urgent need for effective management. Pain, whether acute or chronic, is a complex neurochemical process. Its alleviation depends on the type, with nonopioid analgesics effective for mild to moderate pain, such as musculoskeletal or inflammatory pain, while neuropathic pain responds best to anticonvulsants, tricyclic antidepressants, or serotonin/norepinephrine reuptake inhibitors. For severe acute or chronic pain, opioids may be...
584
Pain
475
Pain serves as a critical warning signal that alerts the body to potential or actual harm. When mechanical pressure on the skin is intense, such as from a sharp pinch, the sensation transitions from touch to pain. Similarly, extreme temperatures, like a hot pot handle, convert the sensation of heat into pain. Pain can also result from overstimulation of other senses, such as blinding light, loud noise, or the intense heat from habañero peppers. This ability to sense pain is essential for...
475