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在感染性角质炎的诊断中应用元基因组下一代测序

Xin-Yu Pan¹, Meng Wang¹, Yi-Dan Xu¹

¹Department of Ophthalmology, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu Province 210006, China.

Journal of ophthalmology

|May 8, 2024

概括

下一代元基因组测序 (mNGS) 提供了在传染性角质炎 (IK) 中更好的细菌和病毒病原体检测. 这种先进的技术有助于诊断混合感染,改善这种严重的眼睛疾病的治疗结果.

科学领域:

眼科医生眼科眼科
传染性疾病传染性疾病
基因组学就是基因组学.

背景情况:

传染性角膜炎 (IK) 对视力构成重大威胁,创伤和异物是主要原因.
准确及时诊断病原体对于有效治疗和视力恢复至关重要.

研究的目的:

评估下一代元基因组测序 (mNGS) 在传染性角质炎 (IK) 的诊断优势.
将mNGS的疗效与传统的诊断方法进行比较,以识别IK中的各种病原体.

主要方法:

在2018年8月至2022年12月期间接受治疗的287名IK患者的回顾性分析.
使用mNGS,分泌物培养,角膜激光共聚焦显微镜 (CM) 和病理切片检测细菌,真菌和病毒感染的检测率的比较.

主要成果:

与其他方法相比,mNGS在细菌和病毒检测方面显示出更高的阳性率.
线状真菌 (例如,Fusarium,Aspergillus) 是常见的,与细菌 (例如,Pseudomonas aeruginosa) 和病毒 (例如,HSV/VZV) 一起.
IK治疗的整体疗效率为92.3%,mNGS在诊断混合感染方面被证明是有效的.

结论:

创伤和异物是传染性角膜炎的主要原因.
下一代元基因组测序 (mNGS) 是一种有效和全面的诊断工具,用于IK的细菌和病毒感染,特别是混合感染.

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