在Senecavirus A VP2蛋白的"膨胀"循环上确定线性B细胞表位,该蛋白参与受体结合
Hanrong Zhou1, Mingxia Sun1, Shibo Su1
1State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang, China.
Frontiers in microbiology
|May 8, 2024
概括
研究人员在塞内卡病毒A (SVA) VP2蛋白上确定了关键的B细胞表位. 这些发现对于开发诊断工具和针对这种猪病原体的以表位基SVA标志物疫苗至关重要.
科学领域:
- 兽医病毒学 兽医病毒学
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
背景情况:
- 塞内卡病毒A (SVA) 是一种新兴的猪病原体,导致小猪囊泡性疾病和死亡.
- SVA的VP2蛋白是抗体生产的目标,对于疫苗和诊断开发至关重要.
研究的目的:
- 在SVA VP2上识别B细胞表位 (BCE) 以开发基于表位的SVA标记疫苗.
- 以诊断和疫苗应用中的潜力来描述已识别的BCE.
主要方法:
- 产生针对SVA VP2.2的单克隆抗体 (mAbs).
- 片扫描和氨酸扫描分析以识别和表征线性BCE.
- 突变分析以确认抗体识别中的表皮质功能.
主要成果:
- 发现了两种新的线性 BCE,即177SLGTYYR183和266SPYFNGL272.
- 由mAb 1B8识别的Epitope177SLGTYYR183是表面暴露的,高度连续的,位于VP2 EF循环的"膨胀"区域.
- 在177SLGTYYR183中的关键氨基酸残留物参与了受体结合,而Y182A突变取消了mAb 1B8的识别.
结论:
- 已识别的ECB,特别是177SLGTYYR183,是开发诊断工具的有价值的目标.
- 这些BCE为构建针对塞内卡病毒A的以表位基标记疫苗提供了基础.
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