肠道微生物新陈代谢与循环mRNA的潜在关联 基于胎儿生长限制中的多基因组测序分析
Hui Tang1, Dan Li1, Jing Peng1
1Hunan Provincial Key Laboratory of Regional Hereditary Birth Defects Prevention and Control, Changsha Hospital for Maternal and Child Health Care Affiliated to Hunan Normal University, Changsha, China.
Mediators of inflammation
|May 8, 2024
概括
胎儿生长限制 (FGR) 涉及改变的肠道微生物群,循环的mRNA和代谢物. 这些因素可能会调解FGR中肠道和循环系统之间的相互作用,提供诊断见解.
科学领域:
- 基因组学和微生物组学
- 代谢学 代谢学 代谢学
- 生殖医学 生殖医学
背景情况:
- 胎儿生长限制 (FGR) 是导致不良妊娠和发育结果的主要原因.
- 导致FGR的确切病理机制在很大程度上是未知的.
- 了解FGR的分子基础对于改善诊断和管理至关重要.
研究的目的:
- 通过使用多组学测序,研究mRNA,肠道微生物群和FGR中的代谢物之间的相互作用.
- 确定与FGR相关的潜在生物标志物和分子机制.
- 建立FGR诊断和理解的理论基础.
主要方法:
- 多组学方法包括RNA测序,16SrRNA测序和代谢学.
- 从健康和FGR怀孕中分析带血,母体血清,便和胎盘组织.
- 使用RT-qPCR,ELISA或西式涂料验证关键mRNA表达的验证.
主要成果:
- 在FGR中观察到循环mRNA配置文件 (例如TRIM34,SMOX,FAM83A,NAPG) 的显著变化.
- 在FGR群中的特定肠道细菌 (如Bacteroides,Akkermansia,Tyzzerella) 的丰富.
- 确定了变化的代谢物水平 (例如,甲氨酸,氨酸) 以及它们与mRNA和微生物群的相关性.
结论:
- 特定的循环mRNA,肠道微生物群和代谢物与FGR有关.
- 代谢物 (甲氨酸,氨酸),微生物群 (Tyzzerella) 和mRNA (TRIM34,SMOX,FAM83A,NAPG) 可能起到FGR中的调解作用.
- 这些发现为了解FGR的肠-循环系统相互作用提供了基础.
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