使用化学衍生和差分移动性光谱学分离安非他胺和甲胺的奇拉性异构体
J Larry Campbell1,2,3,4, Amol Kafle1, Zack Bowman2,5
1SCIEX Concord Ontario Canada.
Analytical science advances
|May 8, 2024
概括
本研究引入了使用差分流动性光谱法 (FIA-DMS) 进行快速流注分析的方法,用于分离和量化安非他命和甲胺的反体. 这种方法提供了一种更快的替代方法,可以与传统的手术液体色谱 (手术LC) 相比较准确.
科学领域:
- 分析化学 分析化学
- 分离科学 分离科学
- 质谱测量质量谱测量
背景情况:
- 奇拉化合物分离,特别是反体,是分析化学的一个重大挑战.
- 在质谱 (MS) 分析之前,通常使用传统方法,如状液体色谱 (LC),气体色谱 (GC) 和毛细血管电泳 (CE).
- 这些方法可能耗时,需要更快的分析技术.
研究的目的:
- 开发和验证一种快速的方法来分离和量化安非他命和甲非他命的反体.
- 为了比较差分移动光谱法 (DMS) 与传统的LC方法进行奇拉分析的性能.
- 为了证明流注分析与DMS (FIA-DMS) 结合用于治疗性药物分析的实用性.
主要方法:
- 异构体对的化学衍生 (R-和S-胺,R-和S-甲胺).
- 使用微分移动性谱法 (DMS) 与质谱法 (MS) 结合的分离和量化.
- 与逆相LC分离进行比较.
主要成果:
- 使用FIA-DMS成功分离和定量R-和S-安非他胺和R-和S-甲胺.
- FIA-DMS在大约1.5分钟内实现了分离,比LC所需的5分钟快得多.
- FIA-DMS证明了与LC方法相当的定量指标 (1-1000 ng/mL范围).
- 在DMS中,分离机制依赖于分析离子和酸修饰剂分子之间的差异性结合能.
结论:
- FIA-DMS是一种快速有效的技术,用于分离和定量安非他命的反体.
- 这种方法为分析这些化合物提供了比传统的性LC更快的替代方案.
- 基于DMS的方法提供了与LC可比的定量性能,使其适用于高通量分析应用.
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