在传统和现代肉中表达与乳腺屏障功能相关的基因表达
K R Kpodo1, K B Miska1, L L Schreier1
1Animal Biosciences and Biotechnology Laboratory, Agricultural Research Service, United States Department of Agriculture, Beltsville, MD, USA.
British poultry science
|May 8, 2024
概括
这项研究研究了不同生长速度的的肠道屏障基因表达. 结果表明,虽然基因表达依赖于年龄,但生长选择可能不会显著改变结合综合体或免疫防御基因.
科学领域:
- 动物科学动物科学
- 遗传学 遗传学 是一个
- 禽类生理学 禽类生理学
背景情况:
- 肠道屏障的完整性对于家禽的健康和表现至关重要.
- 了解与肠道屏障功能相关的基因表达可以为繁殖策略提供信息.
- 品种之间生长速度的差异可能与肠道基因表达的变化有关.
研究的目的:
- 为了比较快速,中等和缓慢生长的中编码肠道屏障蛋白的基因表达.
- 分析关键肠道屏障基因从胚胎发育到化后35天的时间表达模式.
- 调查生长选择对肠道屏障基因表达的潜在影响.
主要方法:
- 六种品种 (ACRB,LHR,RedBro,HH1,Cobb500,Ross708) 被养了35天时间.
- 在多个时间点采集了皮质样本:胚胎日E19,化,以及化后的7,14,21,28,35天.
- 量化PCR被用来评估紧结蛋白 (CLDN,OCLN,ZO,JAM),粘素 (Muc2),免疫球蛋白A (IgA),聚合免疫球蛋白受体 (pIgR) 和脂肪酸结合蛋白 (FABP2) 的基因表达.
主要成果:
- 在品种之间和不同时间点之间观察到克劳丁-1,克劳丁-5,奥克卢丁和ZO-1表达的显著变化.
- 紧结基因表达,除了例外,在化和早期的化后阶段通常是下调的.
- 菌素2 (Muc2) 和免疫球蛋白A (IgA) 表达显示出特定于品种的差异和时间变化,IgA在所有品种中在第14天增加.
结论:
- 的肠道屏障基因表达受到年龄的显著影响.
- 目前的证据表明,对生长的选择可能没有实质性地改变结合复合体和免疫防御基因的表达.
- 需要进一步的研究,以充分阐明在家禽中生长选择和肠道屏障功能之间的关系.
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