情绪压力诱导了老鼠淋巴细胞对随后的电离辐射暴露的适应性反应
P E Umryukhin1,2, E N Mikheeva3, U M Mishina3
1Research Centre for Medical Genetics, Moscow, Russia. pavelum@mail.ru.
Bulletin of experimental biology and medicine
|May 8, 2024
概括
情绪压力使老鼠淋巴细胞适应辐射,模仿适应性反应. 然而,在随后的辐射中,先前的应激暴露矛盾地降低了DNA氧化和保护性NRF2水平.
科学领域:
- 分子生物学分子生物学
- 辐射生物学 辐射生物学
- 压力生理学 压力生理学
背景情况:
- 情绪压力可以影响细胞对外部刺激的反应.
- 淋巴细胞在应激和辐射的适应性反应中发挥作用.
- NRF2和NOX4蛋白质与细胞应激适应有关.
研究的目的:
- 研究大鼠淋巴细胞中情绪压力和电离辐射之间的交叉适应的分子机制.
- 分析慢性和急性情绪应激对氧化应激,细胞死亡和淋巴细胞中关键蛋白质水平 (NRF2,NOX4) 的影响.
主要方法:
- 从遭受慢性 (CES) 和急性 (AES) 情绪压力的老鼠中分离淋巴细胞.
- 对氧化DNA损伤标记物的分析.
- 对淋巴细胞细胞死亡的评估.
- 通过分子分析量化NRF2和NOX4蛋白水平.
主要成果:
- 情绪压力诱导了淋巴细胞的适应反应,其特征是氧化DNA增加,细胞死亡以及NOX4和NRF2蛋白水平升高.
- 对情绪压力的预暴露导致了DNA氧化抑制和辐射后淋巴细胞中NRF2水平的降低.
- 暴露于压力的淋巴细胞在辐射后表现出保护因子NRF2的降低和细胞死亡模式的改变.
结论:
- 情绪压力可以诱导淋巴细胞的适应反应,与辐射诱导的适应有相似之处.
- 以前的情绪应激暴露似乎会损害淋巴细胞对随后的电离辐射的适应和保护机制.
- 淋巴细胞中压力,NRF2,NOX4和DNA损伤之间的相互作用需要进一步研究,以了解放射电阻.
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