来自玉米焦油点病原体Phyllachora maydis的病毒性蛋白质的基因组信息化热带分类和功能性表征
Abigail Rogers1, Namrata Jaiswal2, Emily Roggenkamp3
1Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN 47907.
Phytopathology
|May 8, 2024
概括
菲拉科拉梅迪斯 (Phyllachora maydis) 是一种玉米焦油斑真菌,很可能以生物养为食,并使用特定的效应蛋白来抑制植物免疫力,帮助疾病发展. 这项研究预测了它的生活方式,并描述了关键的影响因素.
科学领域:
- 植物病理学 植物病理学
- 菌群学 菌群学 菌群学是指菌群学
- 分子植物-微生物相互作用
背景情况:
- 菲拉科拉梅迪斯 (Phyllachora maydis) 在玉米中引起经济上显著的焦油斑病.
- 对于P. maydis的营养生活方式和效应蛋白功能的理解有限.
- 描述这些方面对于制定有效的疾病管理策略至关重要.
研究的目的:
- 通过基因组信息的方法预测P. maydis的营养生活方式.
- 为了功能性地描述参与P. maydis病原性中的候选效应蛋白质.
- 阐明在玉米中脱点疾病发展背后的分子机制.
主要方法:
- 基因组注释和CATAStrophy管道被用来预测碳水化合物活性酶 (CAZyme) 的库存.
- 在疾病期间表达的候选效应蛋白 (18) 被选择用于功能分析.
- 使用AlphaFold2和Foldseek评估了结构相似性;通过Nicotiana benthamiana的短暂表达评估了效应器功能,包括监测反应性氧物种 (ROS) 生产.
主要成果:
- 梅迪斯 possesses一个CAZyme剧目与一个生物养的生活方式一致.
- 一个效应器 (PM02_g1115) 与Verticillium dahlia的效应器具有结构上的相似性.
- 两个因子 (PM02_g378,PM02_g2610) 定位在细胞质中,三个因子 (PM02_g1115,PM02_g7882,PM02_g8240) 抑制了基诱导的ROS产生.
结论:
- 这项研究提供了对P. maydis.预测的生物变异性质的见解.
- 几种候选效应蛋白的功能特征,揭示了在抑制植物免疫力中的作用.
- 这些发现为进一步研究焦油斑病的分子基础铺平了道路.
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