温度和产卵剂对野生雌性 (Leuciscus aspius) 在受控条件下的生殖效率的影响
Radosław Król1, Joanna Nowosad2, Krzysztof Kupren3
1Department of Ichthyology and Aquaculture, Faculty of Animal Bioengineering, University of Warmia and Mazury, Olsztyn 10-719, Poland.
Animal reproduction science
|May 8, 2024
概括
优化 (Leuciscus aspius) 的人工繁殖需要特定的温度和荷尔蒙治疗. 水温在10-12°C之间,Ovaprim或Ovopel/Ovaprim组合为排卵和胚胎存活提供了最好的结果.
科学领域:
- 水产养殖和鱼类学
- 鱼类生殖生物学 鱼类生殖生物学
- 保护水产养殖水产养殖的保护
背景情况:
- 人工繁殖对于生产淡水鱼的养殖材料至关重要,但对许多物种来说,包括 (Leuciscus aspius) 在内的协议很少.
- 随着天然鱼种群的减少,需要重新填补种群,需要在受控条件下生产的高质量,生物多样性和健康的养殖材料.
- 现有的关于鱼人工繁殖的研究是有限的,缺乏为繁殖定义的最佳环境和荷尔蒙参数.
研究的目的:
- 开发一个优化的协议,用于 (Leuciscus aspius) 的人工繁殖.
- 确定最有效的热条件和激素刺激,以成功繁殖.
- 加强用于鱼保护和水产养殖的高质量养殖材料的生产.
主要方法:
- 实验I:评估了恒定水温 (10.0,12.0和14.0°C) 对ASP人工繁殖的有效性.
- 实验II:评估产卵剂 (Ovopel,Ovaprim或组合) 对鱼繁殖的疗效.
- 测量了包括排卵率,胚胎存活率和化成功在内的关键生殖指数;分析了卵巢液的pH值作为质量指标.
主要成果:
- 确定最终卵细胞成熟 (FOM) 和asp人工繁殖的最佳水温为10-12°C.
- 使用Ovaprim或Ovopel和Ovaprim组合的激素刺激显著改善了在10-12°C的排卵率和胚胎存活率.
- 卵巢液pH值在8.0-8.4之间表明了生物卵子质量,在pH值7.4以下没有观察到可活胚胎.
结论:
- 控制10-12°C的水温和特定的激素治疗 (Ovaprim或Ovopel/Ovaprim组合) 对于成功的人工繁殖至关重要.
- 开发的协议可以通过生产高品质的鱼养材料来支持补给工作,有助于维持自然种群水平.
- 卵巢液的pH值作为一个有用的初步指标,用于评估卵子活力在asp.
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