在高介电常数基板上检测蛋白质微阵列的无标签OIRD检测,具有增强的内在灵敏度
Meng Li1, Xiaoyi Li1, Dandan Ji1
1Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, School of Materials and Energy, Southwest University, Chongqing 400715, China.
Talanta
|May 8, 2024
概括
基板的介电常数显著影响蛋白质微阵列中的斜发射反射率差异 (OIRD) 灵敏度. 更高的介电常数基板,如,提高OIRD性能,用于无标签的检测.
科学领域:
- 生物光子学 生物光子学
- 表面科学是一门学科.
- 生物感应是一种生物感应.
背景情况:
- 斜发射反射率差 (OIRD) 是一种敏感的技术,用于无标签的蛋白质微阵列检测.
- 基板介电常数对OIRD灵敏性的影响仍然未被充分研究,尽管它与各种材料的兼容性.
研究的目的:
- 为了研究基板介电常数和OIRD灵敏度在顶端事件配置之间的关系.
- 通过理解基板效应来优化基于OIRD的无标签微阵列.
主要方法:
- 结合了理论光学建模与实验评估.
- 使用的玻璃,添加氧化锡 (FTO) 和 (Si) 基板具有不同的介电常数.
- 评估了使用斯特雷普塔维丁作为模型目标和芯片上ELISA进行量化检测的检测性能.
主要成果:
- 光学建模预测,随着基底介电常数的增加,灵敏度更高.
- 实验结果证实了这一趋势,基板显示出最高的灵敏度.
- 通过使用基于的微阵列,达到了斯特雷普塔维丁的5ng/mL的检测极限.
- 芯片上ELISA验证了增强的灵敏性是由于基质的介电常数.
结论:
- 基板介电常数是提高OIRD对无标签蛋白质微阵列敏感性的关键因素.
- 高介电常数材料,如,为基于OIRD的生物传感提供了更好的性能.
- 这项研究为开发更敏感的OIRD无标签微阵列检测系统提供了途径.
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