在口腔和口腔前癌症中的BER基因表达:结合方法提出潜在的生物标志物
Kumud Nigam1, Yogendra Verma2, Manish Dwivedi1
1Amity Institute of Biotechnology, Amity University Uttar Pradesh, Lucknow, Gomti Nagar Ext. Lucknow-226028, India.
Current problems in cancer
|May 8, 2024
概括
在口腔癌和癌前病变中,DNA修复基因XRCC3,NBS1和OGG1的高表达表明它们作为生物标志物的潜力. 这些基因的遗传变异可能会影响它们的表达水平,可能会影响口腔癌的风险.
科学领域:
- 遗传学 遗传学 是一个
- 分子生物学分子生物学
- 癌症研究 癌症研究
背景情况:
- 修复DNA的基因变异与口腔癌风险的改变有关.
- 了解关键DNA修复基因的表达和遗传变异对于口腔癌研究至关重要.
研究的目的:
- 研究口腔癌和癌前疾病中XRCC3,NBS1和OGG1基因的表达水平.
- 分析这些基因中的特定遗传多态 (SNP) 与它们的表达水平之间的相关性.
- 探索这些多形态对mRNA二次结构的影响.
主要方法:
- 实时定量PCR (RT-PCR) 用于测量口腔癌,口腔前癌和对照组的基因表达.
- 聚合酶链反应-限制片段长度多态 (PCR-RFLP) 用于XRCC3,NBS1和OGG1多态的基因定型.
- 用RNAfold软件对研究SNP周围的mRNA序列进行本地化的折叠结构分析.
主要成果:
- 与对照组相比,XRCC3,NBS1和OGG1的转录在口腔癌和口腔癌前组都显著上调.
- 与CC基因型相比,OGG1 GG基因型的转录水平显著增加 (p=0.009).
- 在XRCC3 (C>T) 和OGG1 (C>G) 中的多态性与mRNA二次结构的改变有关,由折叠能量的变化表明.
结论:
- 增加XRCC3,NBS1和OGG1的表达表明它们作为口腔癌和癌前病变的生物标志物的潜力.
- 这些基因中的单核酸多态 (SNP) 可以影响它们的表达,可能是通过对mRNA二级结构的修改.
- 由于样本规模较小,该研究的结果具有暗示性,因此需要进行更大规模的研究来验证.
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