接受定时人工授精协议的原始雌性Bos indicus奶牛的生物化学特征和卵细胞质量
Miguel Pizzolante Bottino1, Laís Reis Carvalho1, Raphael Evangelista Orlandi1
1Department of Veterinary Medicine, UFLA, Lavras, MG, Brazil.
Theriogenology
|May 8, 2024
概括
原始性Bos indicus奶牛对排卵同步的反应较低,原因是胰岛素,IGF-1,胆固醇和葡萄糖的血液度较高. 卵细胞质量和怀孕率在原生母牛和多生母牛之间是相似的.
科学领域:
- 生殖生物学 生殖生物学
- 动物科学动物科学
- 内分泌学 在内分泌学.
背景情况:
- 卵性Bos indicus奶牛对排卵同步协议的反应有所减少.
- 了解潜在原因对于提高牛的生殖效率至关重要.
研究的目的:
- 为了研究生化特征,卵细胞和卵泡细胞质量,以及在经过排卵同步的初卵性和多卵性Bos indicus奶牛的怀孕结果.
- 确定导致第一个小公牛母牛反应较低的因素.
主要方法:
- 进行了两项实验,涉及卵子取卵 (OPU),激素治疗 (雌二和孕激素) 和卵泡吸收.
- 在血液和毛囊液中分析了生物化学概况 (葡萄糖,胆固醇,NEFA,IGF-1,胰岛素).
- 怀孕率是根据一个不同的排卵同步协议来评估的.
主要成果:
- 卵细胞质量和颗粒细胞中的基因表达在平价组之间没有差异.
- 多双的奶牛有更多的卵泡和更大的主导卵泡,但原始双的奶牛有更多的退化的卵细胞.
- 原始牛的血液中胰岛素,IGF-1,总胆固醇和葡萄糖的度较高,特别是在实验2的第10天.
结论:
- 虽然卵细胞质量和怀孕率是可比的,但初卵母牛和多卵母牛之间存在生化差异.
- 血中IGF-1,胆固醇和葡萄糖的升高在原生母牛中可能会影响它们对同步协议的反应.
- 需要进一步研究,以阐明这些代谢物与Bos indicus公牛的繁殖表现相关的确切机制.
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