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大量和单细胞转录组概况识别了长非编码RNAGas5在纤维化中的潜在细胞标

Xiang Zhang¹, Shouci Hu¹, Xiaojun Xiang¹

¹Department of Nephrology, The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine), Hangzhou, China.

Biochimica et biophysica acta. Molecular basis of disease

|May 8, 2024

概括

长非编码RNA Gas5 (lncRNA Gas5) 的损失会使纤维化和损伤恶化. 这表明lncRNA Gas5是慢性病进展的潜在生物标志物和治疗标.

科学领域:

腎臟病學 (nephrology) 是一種醫學.
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

长非编码RNAs (lncRNAs) 在病中发挥作用.
增长停止特异性5 (Gas5) 是一种涉及病态的lncRNA.
它在慢性病 (CKD) 进展中的作用需要进一步研究.

研究的目的:

为了研究CKD中IncRNA Gas5的表达特征.
确定lncRNA Gas5在纤维化进展中的作用.
评估IncRNA Gas5作为CKD的潜在生物标志物.

主要方法:

单边尿路阻塞 (UUO) 模型在小鼠中诱导纤维化.
评估损伤和细胞外基质沉积.
过氧体增殖器激活受体 (PPAR) 信号通路的分析.
单细胞RNA测序 (scRNA-seq) 用于分析纤维细胞转录组学.

主要成果:

失去了 lncRNA Gas5 加剧了UUO诱导的损伤和纤维化.
删除Gas5导致PPAR信号通路的激活,并改变了细胞因子的配置.
scRNA-seq揭示了Gas5删除诱导的异质纤维细胞状态促进纤维化.
lncRNA Gas5调节免疫细胞分化/激活和PPAR通路基因.

结论:

lncRNA Gas5在纤维化中起着保护作用.
缺Gas5会通过激活PPAR信号和重编程免疫细胞,加剧损伤.
lncRNA Gas5是一种潜在的诊断生物标志物和CKD的治疗点.

关键词:

免疫细胞的分化和激活.在PPAR信号传输中.患有脏纤维化单边尿路阻塞单边尿路阻塞在cRNA Gas5 中,

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