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在一次性屏幕印刷碳电极上使用分子打印的聚3,4-乙烯二氧化) 进行细胞染色体c电化学检测

Ganesan Kaniraja¹, Murugesan Karthikeyan¹, Marimuthu Dhinesh Kumar¹

¹Department of Chemistry, Virudhunagar Hindu Nadars' Senthikumara Nadar College (Autonomous & Affiliated to Madurai Kamaraj University), Virudhunagar, 626 001, Tamil Nadu, India.

Analytical biochemistry

|May 8, 2024

概括

这项研究开发了一种用于检测细胞染色体c (cyt c) 的新型传感器,该传感器是亡中的关键蛋白质. 分子印记聚合物传感器提供了一种低成本,高度敏感的方法来检测生物样本中的细胞.

科学领域:

电化学电化学电化学
生物感应是一种生物感应.
材料科学材料科学材料科学

背景情况:

细胞染色体c (cyt c) 在亡中至关重要,但其检测需要敏感的方法.
现有的细胞检测方法可能很复杂或昂贵.

研究的目的:

开发一种分子印记导电聚合物传感器,用于敏感和选择性细胞染色体c检测.
创建一个低成本,高效的平台,用于非侵入性细胞死亡检测.

主要方法:

在印碳电极上 (SPCE) 通过电聚合制造分子印制的聚-3,4-乙烯二氧化 (MIPEDOT).
使用循环电压测量 (CV),电化学阻抗光谱 (EIS),微分脉冲电压测量 (DPV) 和扫描电子显微镜 (SEM) 的表征.
在特定的潜在范围内使用CV对细胞的量化.

主要成果:

MIPEDOT 传感器表现出极好的细胞c.c.分子识别能力.
从1到1200 pM观察到峰值电流和细胞度之间的线性关系.
在高灵敏度下实现了0.5 pM的非常低的检测极限.
在人体血样本中成功检测了cytc,证实了传感器的适用性.

结论:

开发的MIPEDOT/SPCE传感器是一种低成本,简单,高效的细胞检测平台.
这种传感平台显示了提高可靠和精确的非侵入性细胞死亡检测的巨大潜力.
传感器的高灵敏度和选择性使其成为未来生物医学应用的可行工具.

关键词:

3,4-乙烯二氧化四氧化.循环电压计是循环电压计.细胞染色体c 在电化学检测电化学检测分子印记聚合物聚合物幕打印碳电极的使用.

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