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在小鼠中对TM9SF家族成员表达模式的比较分析

Rui Zhao¹, Wenxiong Liao², Duo Tan²

¹Guangdong Second Provincial General Hospital, Guangzhou, 510317, China; Key Laboratory of Brain, Cognition and Education Sciences, Ministry of Education, China; Institute for Brain Research and Rehabilitation, South China Normal University, 510631, Guangzhou, China.

Gene expression patterns : GEP

|May 8, 2024

概括

跨膜9超级家族蛋白 (TM9SFs) 在人体组织中得到广泛的表达. 在小鼠中,TM9SFs表现出偏好的肺表达,TM9SF1在大脑中被丰富并受到发育调节.

科学领域:

细胞生物学细胞生物学
发展生物学发展生物学
遗传学遗传学是一个

背景情况:

跨膜9超级家族蛋白 (TM9SFs) 已被保存,但它们的发育作用在很大程度上仍未知.
以前的研究表明TM9SFs参与了自和分泌途径.

研究的目的:

在人类和小鼠模型中描述TM9SFs的基因和蛋白质表达模式.
为了研究TM9SFs在发育过程中的体内功能.

主要方法:

利用开源数据库,结构预测,免疫光和西方斑点分析.
分析了人类组织表达的RNA测序和蛋白质组学数据.
在小鼠中枢神经系统和视网膜中检查TM9SF表达.

主要成果:

TM9SFs在人体组织中无处不在表达.
鼠标TM9SFs主要在肺部表达,除了TM9SF1,它在大脑中被丰富.
TM9SF1在小鼠大脑中显示出发育上调,而TM9SF蛋白质在神经元和微质中发现,而不是在星球细胞中.
TM9SF3表现出独特的核定位,与其他定位在等离子体膜/细胞质的TM9SF不同.
TM9SF家族成员在视网膜层中表达,主要是在视网膜细胞的血膜上.

结论:

与人类相比,TM9SFs在小鼠中表现出不同的表达模式,这表明器官特定的角色.
TM9SF1可能在神经发育中发挥关键作用,而TM9SF2/3/4可能参与肺功能.
TM9SF3在神经元和微质细胞中具有独特的细胞功能.

关键词:

中枢神经系统中枢神经系统表达方式表达模式.视网膜 (retina) 是一个视网膜.在TM9SF家族.

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