一个灵活的自助式电化学传感器Co-NC/PS@CC用于实时检测细胞释放的H2O2
Yiyi Liu1, Cuncun Wang1, Yong Zhang1
1Key Laboratory for Biorheological Science and Technology of Ministry of Education, College of Chongqing University, Chongqing, 400044, PR China.
Analytica chimica acta
|May 8, 2024
概括
研究人员开发了一种灵活的传感器,使用单原子催化剂来检测活细胞中的过氧化 (H2O2). 这一进步为实时细胞分析和潜在疾病诊断提供了一个新的工具.
科学领域:
- 生物医学工程 生物医学工程
- 纳米技术 纳米技术
- 分析化学 分析化学
背景情况:
- 过氧化 (H2O2) 是一个关键的反应性氧物种 (ROS),参与细胞过程.
- 实时监测活细胞中的H2O2对于了解细胞功能和诊断疾病至关重要.
研究的目的:
- 开发一个灵活的,自我支持的传感平台,用于实时检测活细胞中的H2O2.
- 使用单原子催化剂提高传感器性能.
主要方法:
- 碳布 (CC) 用作灵活传感平台的基板.
- 一个单原子纳米酶 (Co-NC/PS) 通过P和S兴奋剂为过氧化酶类活性准备.
- 对传感器的H2O2检测性能进行了评估,包括其响应范围,检测极限和稳定性.
主要成果:
- 该Co-NC/PS@CC传感器表现出极好的灵活性,导电性和吸附性质.
- 对H2O2 (117328μM) 实现了广泛的线性响应范围和低检测极限 (0.1687μM).
- 传感器表现出良好的可重复性,可重复性,抗干扰性和稳定性.
结论:
- 开发的Co-NC/PS@CC传感器成功地被应用于检测MCF-7活细胞分泌的H2O2.
- 这项工作扩大了单原子纳米酶在活细胞生物传感中的应用.
- 这些发现对健康监测和临床诊断有重大影响.
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