开发可切换的CO2基于溶剂的深度环氧化液体液体微提取方法:在尿液分泌和组织分布研究中的应用
Lin Yang1, Xiaoyue Yang1, Erxu Wang1
1Hebei Key Laboratory of Organic Functional Molecules, College of Chemistry and Materials Science, Hebei Normal University, Shijiazhuang, Hebei, 050024, China.
Analytica chimica acta
|May 8, 2024
概括
本研究介绍了一种新的,环保的样品制备方法,使用可转换二氧化碳的深层欧特溶剂 (DES) 来更快,更准确地分析生物样品. 该技术增强了药物开发和药理动力学研究.
科学领域:
- 分析化学 分析化学
- 药理学 药理学是指药理学的学科.
- 绿色化学 绿色化学
背景情况:
- 药理动力学研究对于药物开发至关重要,需要有效的样本准备.
- 传统方法使用有毒溶剂,耗时.
- 深度环氧溶剂 (DES) 提供了一个环保,高效的替代品.
研究的目的:
- 为生物样品开发一种新,快速和灵敏的样品预处理方法.
- 在液体-液体微提取 (LLME) 中利用可转换CO2的DES.
- 为了分析Nelumbinis plumula (NP) 提取物的药动力学概况.
主要方法:
- 建立了一种使用可切换DES的液体-液体微提取 (LLME) 方法.
- 优化了关键参数,包括DES类型,体积,pH值和提取时间.
- 使用高性能液体染色学-并联质谱法 (HPLC-MS/MS) 分析的样品.
主要成果:
- 优化的方法显示出极好的线性 (r2 ≥0.9958),精度 (RSD ≤6.01%),恢复 (52.44%-98.12%) 和矩阵效应 (86.22%-119.30%).
- 分析了NP类化合物的尿排泄和组织分布 (liensinine,isoliensinine,neferine).
- 确定累积的尿排泄和确定肝脏,脏和脏的初级分布.
结论:
- 开发了一种简单,快速,经济高效,环保的样本准备技术,使用可切换二氧化碳的DES-LLME.
- 集成去蛋白化和干扰分子去除在一个单一的步骤.
- 提供了使用DES-LLME的NP化物尿液分泌和组织分布的第一份报告,提供了对草药体内生物行为的见解.
相关概念视频
01:30Capillary Electrophoresis: Applications
393
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
393
00:56Extraction: Advanced Methods
446
Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is...
446
01:10Drug Excretion: Miscellaneous Routes
65
Drug excretion involves various organs, including the liver, intestines, skin, and eyes. In the case of drugs or toxins, they can be actively secreted into bile by transporters in the hepatocyte's canalicular membrane. These substances enter the GI tract during digestion and may be reabsorbed into the body from the intestine. This process, known as enterohepatic recycling, can significantly prolong the presence and effects of a substance in the body. To interrupt this cycle, specific...
65