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开发一种经过工程改造的Bacillus subtilis菌株,用于无抗生素的糖糖异粒酶生产

Mingyu Li¹, Xiaopeng Ren¹, Ming Xu¹

¹School of Biological Engineering, Dalian Polytechnic University, Dalian, China.

Biotechnology journal

|May 8, 2024

概括

一种经过工程改造的Bacillus subtilis菌株使得无抗生素的糖糖异粒酶 (SIase) 能够产生. 这种系统有效地产生异糖,一种功能糖,为工业应用铺平了道路.

科学领域:

生物技术是生物技术.
酶学是一种酶学.
食品科学食品科学食品科学

背景情况:

糖分异构酶 (SIase) 对于生产异构糖,一种在食品工业中使用的功能糖至关重要.
目前的SIase生产方法由于低效的异质表达系统而面临限制.
需要安全,高效和无抗生素的SIase.生产系统.

研究的目的:

开发一种经过工程改造的Bacillus subtilis菌株,用于无抗生素生产糖糖异粒酶 (SIase).
为了优化SIase表达和酶活性,用于工业应用.
为了评估工程菌株在从糖糖中生产异二醇的效率.

主要方法:

利用CRISPR/Cas9基因编辑修改Bacillus subtilis菌株TEA,创建具有增强蛋白质表达能力的TEA4菌株.
评估了各种构成性和可诱导性促进剂,以确定细胞外SIase产生最有效的系统.
优化培养基条件和使用摇瓶培养来最大限度地提高SIase活动.

主要成果:

经过工程改造的Bacillus subtilis菌株TEA4,利用可诱导马尔的促进剂Poglv,实现了21.7 U mL-1.1的细胞外SIase活性.
在培养基的进一步优化中,在摇动瓶培养中,SIase活性提高到26.4 U mL−1.
来自工程菌株的原始酶成功地从高度的糖糖中产生了83.1%的最大产量的异醇.

结论:

一种工程 Bacillus subtilis 菌株为无抗生素的 SIase 生产提供了安全高效的系统.
开发的系统显示了工业规模生产异二醇的巨大潜力.
这项研究为SIase在食品工业中的更广泛应用铺平了道路.

关键词:

这种细菌是 Bacillus subtilis.糖糖分异构酶是一种糖分异构酶.生物转化反应反应表达方式表达系统表达系统活动主办人活动主办人

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