相关实验视频

Updated: Jun 26, 2025

Customization of Aspergillus niger Morphology Through Addition of Talc Micro Particles

Customization of Aspergillus niger Morphology Through Addition of Talc Micro Particles

Published on: March 15, 2012

通过提高NADPH可用性来加速酸生物合成的Aspergillus niger的代谢工程

Na Wu^1,2, Xingyu Wu², Mingyu Zhang²

¹College of Marine and Bioengineering, Yancheng Institute of Technology, Yancheng, China.

Biotechnology journal

|May 8, 2024

概括

补充Aspergillus niger与NADPH前体增加了11%的L-酸产量. 抑制NAD激酶进一步提高了酸产量和生产率,证明了大量化学合成的可行代谢工程策略.

科学领域:

生物技术是生物技术.
代谢工程是代谢工程.
工业微生物学工业微生物学

背景情况:

L-酸是一种有价值的有机酸,通过可再生资源的微生物发酵产生.
协因子NADPH对于L-酸生物合成和细胞氧化还原平衡至关重要.
优化NADPH的可用性是提高微生物生产产量的关键.

研究的目的:

通过操纵NADPH水平来改善Aspergillus niger中的L-酸生产.
研究NAD激酶 (Utr1p和Yef1p) 在增强细胞质NADPH中的作用.
通过代谢工程来提高L-酸的标位和生产率.

主要方法:

用NADPH前体 (尼古丁胺,尼古丁酸) 补充发酵介质.
基因工程 Aspergillus niger 的过度表达 NAD 激酶 Utr1p 和 Yef1p.
生物化学分析以评估氧化应激和NADPH水平.
优化发酵以确定酸盐度和生产力.

主要成果:

添加NADPH前体增加了11%的L-酸产量.
过度表达Utr1p和Yef1p减少了氧化应激,并增加了NADPH的可用性.
过度表达Utr1p的工程菌株实现了110.72g/L的酸盐标位和1.03g/L/h的生产率.
酸盐度和生产率分别提高了24.5%和44.7%.

结论:

通过NAD酶上调调节来增加细胞质NADPH水平是增强L-酸在Aspergillus niger中的生产的有效策略.
这种代谢工程方法为工业生产L-酸提供了一个有前途的方法.
开发的策略可以潜在地应用于由真菌宿主生产其他散装化学品.

关键词:

黑色阿斯珀吉勒斯 (Aspergillus niger) 是一个在L-酸中.它们是NAD酶和NAD酶.它们是NADPH,NADPH.代谢工程是代谢工程.氧化应激是一种氧化应激.

更多相关视频

Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the α-amylase Inhibitor from Lablab purpureus L.

Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the α-amylase Inhibitor from Lablab purpureus L.

Published on: February 15, 2019

Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production

Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production

Published on: September 20, 2016

相关概念视频

Fates of Pyruvate

Fates of Pyruvate

Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...