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功能转录组分析显示,在杂交牛的慢性热应激中,MAPK-SMAD信号通路的上调

Gaurav Dutta¹, Rani Alex², Ayushi Singh¹

¹Animal Genetics and Breeding, ICAR-National Dairy Research Institute, Karnal, Haryana, 132001, India.

International journal of biometeorology

|May 8, 2024

概括

印度乳制品杂交品种在热应激下表现出改变的基因表达. 这项研究确定了热应激反应的关键基因,这些基因对于改善热带气候下的牲畜弹性和耐热性至关重要.

科学领域:

动物遗传学动物遗传学
分子生物学分子生物学
适应气候变化适应气候变化

背景情况:

由于热带气候条件,印度的牲畜,特别是乳制品杂交品种,非常容易受到热应激.
气候变化加剧了热应激,对印度的畜牧生产力和性构成了重大挑战.

研究的目的:

为了研究印度杂交牛的热应激反应背后的分子机制.
使用全转录组分析识别受热应激影响的差异表达基因 (DEGs) 和相关途径.

主要方法:

全转录组RNA测序 (RNA-Seq) 在热中性和热应激条件下对杂交牛进行.
差异表达基因 (DEGs) 通过日志双变化和错误发现率 < 0.05.05 确定.
使用基因本体学,KEGG,Reactome路径以及g:Profiler和ShinyGO等网络工具进行了功能性注释和路径分析.

主要成果:

在热应激下,总共有468个基因受到上调,2273个基因受到下调.
过度代表的关键途径包括神经元发育,信号传递,MAPK,Smad,Rap 1信号传递,亡和氧化应激.
下调基因主要参与mRNA处理,免疫反应 (B细胞受体,NLR信号传递) 和无意中介衰变 (NMD).

结论:

这项研究提供了印度乳制品杂交品种热应激反应的综合分子概况.
已识别的热应激反应基因为基因选择提供了潜在的目标,以增强气候弹性和耐热性.
了解这些分子通路对于制定有效的策略来减轻气候变化对牲畜的影响至关重要.

关键词:

卡兰炸条卡兰炸条 MAPK和Smad信号通道的信号通道.氧化应激是一种氧化应激.转录组转录组就是一个转录组.在mRNA处理过程中.

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