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相关概念视频

Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

11.1K
Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
11.1K
Heterochromatin02:38

Heterochromatin

12.5K
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...
12.5K
Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

1.6K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
1.6K
Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

8.3K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
8.3K
Euchromatin01:01

Euchromatin

6.9K
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions take up more dye, appearing darker, while the less-compact areas take up less dye and appear lighter. Based on the compaction level, chromatins are classified into two primary forms – euchromatin and heterochromatin.
Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...
6.9K

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相关实验视频

Updated: Jun 26, 2025

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq
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将基因型映射到单细胞中的染色体可访问性概况

Franco Izzo1,2,3,4, Robert M Myers5,6,7,8, Saravanan Ganesan5,6,7

  • 1New York Genome Center, New York, NY, USA. franco.izzo@mssm.edu.

Nature
|May 8, 2024
PubMed
概括

身体突变改变了染色质的可访问性,影响了细胞分化. 一种名为GoT-ChA的新方法揭示了JAK2V617F突变在血干细胞中的表观遗传,

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Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq
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An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues
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相关实验视频

Last Updated: Jun 26, 2025

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科学领域:

  • 表观遗传学和基因组学
  • 血液形成
  • 癌症生物学

背景情况:

  • 染色体可访问性的变化对于细胞分化至关重要.
  • 身体突变会破坏这些模式,导致细胞异常生长.
  • 在人体样本中研究突变对表观基因组的影响是很困难的,因为细胞类型混合.

研究的目的:

  • 开发一种分析人体突变如何影响人类克隆生长中的表观遗传景观的方法.
  • 研究JAK2V617F突变对骨髓增殖性瘤中的染色质可访问性的影响.

主要方法:

  • 开发了具有单细胞染色体可访问性的向基因型 (GoT-ChA),以在单细胞分辨率下将基因型与染色体可访问性联系起来.
  • 将GoT-ChA应用于JAK2V617F突变骨髓增殖瘤患者的CD34+细胞.
  • 扩展平台 (DOGMA-seq) 包括RNA表达和细胞表面蛋白质分析.

主要成果:

  • 在JAK2V617F突变的造血前体中发现了细胞内在和细胞状态特异性的表观遗传变化.
  • 在造血干细胞中观察到的促炎特征和在巨核细胞前代细胞中观察到的益纤维性炎症特征.
  • 证明JAK2V617F突变导致细胞内在,细胞类型特定的表观遗传重新连接.

结论:

  • 像JAK2V617F这样的体内突变会以细胞特异的方式诱导显著的表观遗传变化.
  • 这些表观遗传变化影响着炎症和细胞分化轨迹.
  • GoT-ChA平台提供了一个强大的工具,用于在各种环境中研究体质突变和表观遗传学.