展开 chaetognaths 的腹部神经中心
June F Ordoñez1, Tim Wollesen2
1Unit for Integrative Zoology, Department of Evolutionary Biology, University of Vienna, 1030, Vienna, Austria.
Neural development
|May 9, 2024
概括
这项研究揭示了Spadella cephaloptera中保存的双边神经发生基因,表明神经系统的祖先中侧模式. 柴托格纳斯神经发育为无脊椎动物进化提供了洞察力.
科学领域:
- 发展生物学 发展生物学
- 进化生物学 进化生物学
- 神经科学是一个神经科学.
背景情况:
- 海洋无脊椎动物Chaetognaths具有争论的基因位置和类似于原生体的神经系统.
- 链神经发生的分子机制在很大程度上仍然没有被描述.
- 这项研究调查了Spadella cephaloptera中的神经发生,以了解双边神经系统的发展.
研究的目的:
- 为了检查Spadella cephaloptera胚胎后发育期间关键双边神经发生和神经模式基因的表达特征.
- 为了确定神经模式基因是否在神经中表现出分层的中侧表达,类似于其他双边基因.
主要方法:
- 采用了全式光 in situ 杂交和共聚焦显微镜.
- 分析了神经分化 (elav),模式 (foxA, nkx2.2, pax6, pax3/7, msx) 和神经功能 (ChAT, VAChT) 基因的表达模式.
- 对Spadella cephaloptera幼和早期幼进行了分析.
主要成果:
- 埃拉夫基因表达表明神经区域超出了先前已知的区域,包括腹腔脑.
- 在幼中,神经模式基因在脑和腹部神经中心 (VNC) 显示出明显的表达,在幼中变得更广泛.
- 模式基因的表达域也在消化道和感官器官中发现;ChAT和VAChT局部化为特定的VNC亚群.
结论:
- 双胞胎神经标记基因的同类物体在Spadella cephaloptera神经发生过程中表现出保留的作用.
- 链动物中VNC模式基因的中侧表达模式与其他双边模型并不直接一致.
- 一个横向展开的VNC显示了与保守的双边中侧区域化相似之处,支持双边干神经系统的祖先模式模型.
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