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普雷沃特拉 (Prevotella melaninogenica) 通过肌素轻链激酶破坏了口腔上皮质屏障的功能

Yiting Guo¹, Wenhao Han², Yuan He¹

¹Department of Oral Medicine, Stomatology Hospital and Dental School of Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai, China.

|May 9, 2024

概括

普雷沃特拉黑色素原菌 (Pm) 增加了口腔角质细胞的透性,并通过上调肌酸轻链激酶 (MLCK) 来破坏表皮屏障. 抑制MLCK恢复了屏障功能,这表明PM在口腔平病原发生过程中发挥了作用.

科学领域:

口腔微生物学口腔微生物学
皮质生物学皮质生物学
免疫皮肤学免疫皮肤学

背景情况:

口腔平坦 (OLP) 与口腔粘膜上Prevotella melaninogenica (Pm) 的增加有关.
Pm有能力侵入OLP患者的上皮.
Pm对口腔角质细胞的具体影响尚未完全阐明.

研究的目的:

研究Prevotella melaninogenica (Pm) 对人类口腔角质细胞 (HOKs) 的影响.
识别Pm诱导的上皮质屏障功能障碍背后的分子机制.
在这个过程中探索髓轻链激酶 (MLCK) 的潜在作用.

主要方法:

建立了PM和HOK的共同文化模式.
评估HOK单层透性和封闭区-1 (ZO-1) 表达.
利用RNA测序 (RNA-seq) 来识别差异表达基因 (DEG),专注于MLCK.
用一种MLCK抑制剂 (ML-7) 来评估其对上皮质屏障功能的影响.

主要成果:

与Pm共同培养增加了HOK单层透性,并降低了ZO-1表达.
在HOKs中,Pm证明了细胞内生存率.
在Pm-HOK模型中,RNA-seq将MLCK确定为一个显著上调的DEG.
抑制MLCK逆转了透率的增加,并恢复了ZO-1的表达.

结论:

普雷沃特拉黑色素原菌 (Pm) 破坏了口腔上皮质屏障的功能.
肌酸氨酸轻链激酶 (MLCK) 涉及Pm介导的上皮质屏障破坏.
向MLCK可能通过恢复上皮质完整性为OLP提供治疗策略.

关键词:

普雷沃特拉 (Prevotella melaninogenica) 是一种带有黑色素的植物.在RNA-seqq.肌酸氨酸轻链激酶的作用在口腔中,有平坦的海.

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