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相关概念视频

RNA Editing02:23

RNA Editing

9.0K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.0K
RNA Interference01:23

RNA Interference

26.0K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.0K
Experimental RNAi02:15

Experimental RNAi

6.1K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.1K
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

16.7K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
16.7K
RNA Splicing01:32

RNA Splicing

56.3K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
56.3K
Pre-mRNA Processing: Modification of pre-mRNA Ends01:35

Pre-mRNA Processing: Modification of pre-mRNA Ends

9.3K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
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相关实验视频

Updated: Jun 26, 2025

A Nonsequencing Approach for the Rapid Detection of RNA Editing
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A Nonsequencing Approach for the Rapid Detection of RNA Editing

Published on: April 21, 2022

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使用基于IDR的策略扩展RNA编辑工具包.

Minghui Di1,2, Junjun Lv1,2, Zhengyu Jing1,2

  • 1School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.

Molecular therapy. Nucleic acids
|May 9, 2024
PubMed
概括

研究人员开发了一种新的人源RNA基编辑器,用于C到U编辑. 融合一个富含胺的域提高了它的效率,展示了一种改善RNA基编辑器的一般方法.

关键词:
科尔特斯 (Cirts) 是一个古老的品种.这是一个IDR IDR.这就是LLPS.MT:RNA/DNA编辑 编辑编辑RNA基的编辑紧紧的 紧的 紧的人类起源人类起源免疫性 免疫性 免疫性

更多相关视频

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
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CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

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RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes
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RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes

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相关实验视频

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A Nonsequencing Approach for the Rapid Detection of RNA Editing
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A Nonsequencing Approach for the Rapid Detection of RNA Editing

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CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
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CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

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RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes
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RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes

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科学领域:

  • 分子生物学分子生物学
  • 生物技术是生物技术.
  • 基因工程是一种基因工程.

背景情况:

  • RNA基编辑提供了精确的基因改造,但在C到U变化的效率和特异性方面面临挑战.
  • 现有的编辑器往往缺乏理想的特性,例如紧,高效和非免疫性.

研究的目的:

  • 开发一个紧的,全人类的C到URNA基编辑器.
  • 通过一种新的策略,提高RNA基编辑器的效率.

主要方法:

  • 人类C到U编辑酶RESCUE-S与Cas启发的RNA向系统 (CIRTS) 融合,以创建CIRTS-RESCUEv1 (V1).
  • 从人体CYCT1中加入一个富含histidine的域 (HRD),称为液-液相分离 (LLPS),并将其纳入V1编辑器.
  • 编辑效率和机制的评估,包括依赖LLPS的点形成.

主要成果:

  • 最初的V1编辑器显示效率低.
  • 与HRD的融合显著提高了V1.1的目标编辑效率.
  • 通过HRD介导的增强与液态-液态相分离 (LLPS) 有关,其他内在无序区域 (IDR) 也表现出增强.
  • 人力资源发展融合策略被证明适用于其他C到URNA编辑器.

结论:

  • 一个新的,紧的,人类起源的C到URNA基编辑器 (CIRTS-RESCUEv1) 被开发出来.
  • 纳入内在无序的区域,利用液态-液态相分离,是提高C到URNA基编辑器效率的一般有效策略.
  • 这项工作扩展了RNA编辑工具包,并提供了一种改善编辑器性能的方法.