微RNA的转录是由发育信号通路和转录因子调节的
Malcolm Arnott1, Nina Faye Sampilo1, Jia L Song1
1Department of Biological Sciences, University of Delaware, Newark, DE, United States.
Frontiers in cell and developmental biology
|May 9, 2024
概括
这项研究调查了转录因子和信号通路在海发育过程中如何调节微RNA (miRNA). 我们确定Tbr,Alx1和Ets1是特定miRNA的关键调节者,影响发育过程.
科学领域:
- 发展生物学 发展生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 微RNAs (miRNAs) 对生物过程至关重要,但它们的转录调节是不太了解的.
- 毫米RNAs的失调与疾病和发育缺陷有关.
- 之前的研究表明,在海的发育过程中,miRNA表达具有动态性,这表明了转录控制.
研究的目的:
- 研究在海胚胎发育过程中通过关键信号通路和转录因子对特定miRNA的转录调节.
- 为了确定直接调节miRNA表达的转录因子.
- 为了确定主要信号通路对miRNA转录水平的影响.
主要方法:
- 利用药理学抑制剂,遗传结构和功能丧失试剂来扰乱信号通路和转录因子.
- 评估保存和特定物种miRNAs的转录水平.
- 采用计算方法 (MEME-Suite,FIMO) 来预测转录因子结合位.
主要成果:
- 建议Tbr,Alx1和Ets1作为*Sp*miR-1,*Sp*miR-31和*Sp*miR-71的转录调节剂.
- 在Wnt,Nodal,MAPK和Sonic Hedgehog信号的干扰时观察到对miRNA水平的显著影响.
- 发现Delta/Notch,VEGF或BMP信号干扰对miRNA水平没有显著影响.
结论:
- 提供了关于miRNAs通过信号通路和转录因子在胚胎环境中的转录调节的见解.
- 确定了控制miRNA表达的特定转录因子和信号通路.
- 通过miRNA控制,有助于理解发育过程的遗传调节.
相关概念视频
Regulation of Expression at Multiple Steps
893
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
893
MicroRNAs
3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
Regulation of Expression Occurs at Multiple Steps
22.6K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
22.6K
Master Transcription Regulators
6.9K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.9K
RNA Polymerase II Accessory Proteins
9.2K
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
9.2K
Transcription
21.5K
Transcription is the synthesis of RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in correctly synthesizing messenger RNA (mRNA). Transcriptional regulation is responsible for the differentiation of different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
21.5K


