Cutibacterium acnes侵入前列腺上皮细胞,诱导BRCAness作为前列腺癌的可能病原体
Shingo Ashida1, Chiaki Kawada1, Hiroko Tanaka2
1Department of Urology, Kochi Medical School, Nankoku, Japan.
The Prostate
|May 9, 2024
概括
前列腺癌组织中Cutibacterium acnes (C. acnes) 感染可能会损害DNA修复. 这种细菌感染可能导致BRCAness,增加癌症风险.
科学领域:
- 微生物学 微生物学
- 在瘤学瘤学.
- 遗传学 是一个遗传学.
背景情况:
- 慢性炎症和感染与前列腺癌的发展有关.
- 识别与前列腺癌相关的特定病原体对于了解其病因至关重要.
研究的目的:
- 为了识别与前列腺癌相关的微生物群或病原体.
- 调查Cutibacterium acnes在前列腺癌发病过程中的作用.
主要方法:
- 使用RNA-seq.对20个人类前列腺癌组织的转录组分析.
- 从新组装非人类序列以识别候选微生物群.
- 免疫组织化学检测组织中的C. acnes.
- 在体外感染前列腺上皮细胞的C. acnes.
- 对基因表达,DNA修复途径 (同源重组,Fanconi贫血) 和BRCA2水平的分析.
主要成果:
- 已经确定了四种候选细菌:Moraxella osloensis,未培养的 chroococcidiopsis,Cutibacterium acnes 和微型白菌.
- 在20个前列腺癌样本中,C. acnes在19个样本中检测到.
- 在实验室中,C. acnes感染导致同源重组和Fanconi贫血路径发生显著变化.
- 菌侵入前列腺上皮细胞,并损害同源重组修复,由减少BRCA2表达和改变的γH2AX焦点证明.
结论:
- 前列腺中的C. acnes感染可能会导致同源重组缺陷 (BRCAness).
- 这种缺陷会导致DNA双链断裂,可能会增加前列腺癌的风险.
- 是一种潜在的前列腺癌发展的病因因素.
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