一种药理学方法,用于研究在米 - Magnaporthe oryzae 相互作用中的效应因子转移
Ely Oliveira-Garcia1, Allison Jane Hamilton1
1Department of Plant Pathology and Crop Physiology, Louisiana State University Agricultural Center, Baton Rouge, LA, USA.
Plant signaling & behavior
|May 9, 2024
概括
像Magnaporthe oryzae这样的真菌病原体使用克拉特林介导的内细胞分裂 (CME) 来向植物细胞输送效应蛋白. 抑制CME阻断了效应体内化,揭示了植物和真菌相互作用的关键机制.
科学领域:
- 植物病理学 植物病理学
- 分子植物-微生物相互作用
- 细胞生物学 细胞生物学
背景情况:
- 在感染期间,真菌病原体分泌出效应蛋白来操纵宿主植物.
- 米爆真菌,Magnaporthe oryzae,利用生物介面复合体 (BIC) 进行效应器转移.
- 细胞质效应体通过动态膜效应体区 (MEC) 和克拉介导内细胞分裂 (CME) 转移到宿主细胞.
研究的目的:
- 研究CME在Magnaporthe oryzae中细胞质效应因子转移到米细胞中的作用.
- 确定CME是否对于生物质界面复合体 (BIC) 内的膜状效应区 (MEC) 的形成至关重要.
主要方法:
- 用克拉重链抑制剂 (内西丁-9和内西丁-9-17) 治疗受感染的水细胞.
- 监测光标记的细胞质效应器 (Bas1和Pwl2) 和细胞质效应器 (Bas4) 的内部化.
- 微观分析生物变性界面复合体 (BIC) 和膜状效应区 (MEC) 形态.
主要成果:
- 克拉思林重链抑制剂 (内西丁-9和内西丁-9-17) 阻断了细胞质效应因子Bas1和Pwl2.2的内化.
- 抑制CME导致胀的BIC缺乏MEC.
- 质效应者Bas4局部不受ES9-17治疗的影响,这表明特异性.
结论:
- 在Magnaporthe oryzae中细胞质效应体转位依赖于BIC内的克拉特林介导内细胞 (CME).
- MEC可能是通过CME路径形成的或依赖于CME路径.
- 效应者可能会选择植物内源性内细胞化机制,以成功感染.
关键词:
戈尔吉独立分泌的分泌物马格纳波特 (Magnaporthe oryzae) (同义词) 是一种植物. 皮里库拉里亚 (Pyricularia oryzae) 是一个植物.生物变性界面复合体 (BIC) 是一种生物变性界面复合体.克拉思林重链药物 克拉思林重链药物克拉特林介导的内细胞分裂 (CME)有效者转移的转移恩多西丁9 (ES9) 的使用.恩多西丁9-17 (ES9-17) 的使用.更多相关视频
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