正义转录因子中的功能分歧:来自AtCBF2/3/1和OsDREB1C的见解
Deyin Deng1, Yixin Guo1, Liangyu Guo1
1State Key Laboratory of Subtropical Silviculture, College of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou 311300, China.
Molecular biology and evolution
|May 9, 2024
概括
像C-重复结合因子 (CBFs/DREB1s) 这样的正向转录因子显示大米 (OsDREB1C) 和阿拉比多普西斯 (AtCBF2/3/1) 之间的功能差异. 这种分歧影响了使用效率,光合作用和应激反应,这是由基因调节的进化变化所驱动的.
科学领域:
- 植物分子生物学 植物分子生物学
- 进化基因组学是进化的基因组学.
- 植物生理学 植物生理学
背景情况:
- 正义基因传统上假定保留功能,但证据表明存在分歧.
- C-重复结合因子/脱水反应元素结合蛋白1s (CBFs/DREB1s) 是寒冷适应的关键,但可以限制生长.
- 米OsDREB1C独特地提高了利用效率 (NUE),光合作用和产量,与典型的CBF/DREB1角色不同.
研究的目的:
- 研究大米OsDREB1C和阿拉比多普西斯AtCBF2/3/1.1.的分离分子机制.
- 探索这些正统的转录因子的进化轨迹,调节体和转录体.
- 了解基因重复和随后的进化如何导致转录因子的功能分歧.
主要方法:
- 在大米和阿拉比多普西斯基因组中对OsDREB1C和AtCBF2/3/1进行比较进化分析.
- 全基因组结合特征分析 (ChIP-seq) 用于确定DNA结合偏好.
- 跨物种的多基因分析 (转录基因组学,表观基因组学) 以确定受调节的基因正统组.
- 对基因增加/损失和表达重编程的生物信息分析.
主要成果:
- OsDREB1C和AtCBF2/3/1形成了不同的进化类,DREB1C类受转子子活动的影响.
- OsDREB1C更喜欢GCCGAC绑定站点,与AtCBF2/3/1的A/GCCGAC偏好不同,因为它在AP2/ERF域中进行了R12替换.
- 多原子分析揭示了由每个因子调节的共享和独特的基因正整组,与特定功能相关 (NUE,OsDREB1C的光合作用;AtCBF2/3/1的激素/压力).
- 显著的基因收益/损失和表达重编程是功能分歧的基础.
结论:
- OsDREB1C和AtCBF2/3/1表现出由明显的监管演变驱动的实质性功能分歧.
- 进化机制,包括基因重复,转子子活性和改变的DNA结合特异性,有助于功能多样化正体转录因子.
- 研究结果提供了基因组规模的洞察力,了解转录因子进化如何塑造植物适应和农业特征.
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