乙氨基细胞的专业化受食道上皮层微环境的暴露所调节
Julia L M Dunn1, Andrea Szep1, Emily Gonzalez Galan1
1Division of Allergy & Immunology, Cincinnati Children's Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229, United States.
Journal of leukocyte biology
|May 9, 2024
概括
埃索诺菲尔食道炎中的明显的埃索诺菲尔子集是由特定的信号塑造的. 益炎和益纤维细胞因子诱导独特的乙素激活配置文件和功能,影响疾病的发病性.
科学领域:
- 免疫学 免疫学 免疫学
- 胃肠病学 胃肠病学
背景情况:
- 氨基酸在健康和疾病中表现出多种功能.
- 了解异性食道炎 (EoE) 中异性食道细胞的专业化至关重要.
研究的目的:
- 为了研究特定的细胞因子 (IL-13和TGF-β) 如何影响人类乙氨基基基的功能和基因表达,在与EoE相关的ex vivo模型中进行研究.
- 确定细胞因子诱导的乙酸酶激活对其他细胞 (如纤维细胞) 的功能后果.
主要方法:
- 在用IL-13或TGF-β刺激下,人类乙氨基酸与食道上皮细胞共同培养.
- 分析了埃索诺菲尔表面标记物表达,细胞因子分泌,细胞外过氧化酶活性和化学反应.
- 与纤维细胞共同培养被用来评估由乙酸酶诱导的纤维细胞激活.
- 翻译研究检查了患者活检样本中的乙氨基标志物表达.
主要成果:
- 刺激IL-13增加了乙氨基基细胞的CD69表达,GRO-α,IL-8和巨细胞殖民地刺激因子的分泌,以及细胞外过氧化酶的活性.
- 刺激TGF-β增加了乙素CD81,CD62L和CD25的表达,IL-6的分泌,以及对CCL11的化学反应.
- 由TGF-β诱导的纤维细胞上调调节SERPINE1和纤维菌素激活的埃索诺菲尔.
- 从EoE患者活检中,CD62L表达在eosinophil上是异质的.
结论:
- 在EoE中,与疾病相关的细胞因子会诱导独特的乙酸酶激活状态和基因表达特征.
- 这些发现凸显了氨酸在EoE病原发生中的复杂作用,并表明了潜在的治疗点.
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