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辅酶Q10在植物性扩展剂的冷保存过程中保持雄鹿的精子质量
Behbood Khazravi1, Mahdi Khodaei-Motlagh1, Reza Masoudi2
1Department of Animal Science, Faculty of Agriculture and Natural Resources, Arak University, Arak 38156-8-8349, Iran.
Animal reproduction science
|May 9, 2024
概括
将辅酶Q10 (CoQ10) 添加到精液延长剂中可以改善解后的精子质量. 10μM CoQ10的最佳度显著提高了运动性,活力,并减少了冷保存期间的氧化损伤.
科学领域:
- 生殖生物学 生殖生物学
- 动物科学动物科学
- 生物化学 生物化学
背景情况:
- 小反动物精液的冷保存对于繁殖计划至关重要.
- 精子冷保存通常会导致由于氧化应激和膜损伤而降低生育能力.
研究的目的:
- 评估大豆莱素扩展剂中可变的辅酶Q10 (CoQ10) 度对冷保存后山羊精液质量的影响.
- 确定最佳的 CoQ10 度,以在冷和解过程中保持雄鹿精子参数.
主要方法:
- 雄鹿精液被稀释在基于大豆莱素的扩展剂中,其CoQ10度为0,0.1,1,10和100微米.
- 解后的精液质量通过评估运动性,膜完整性,线粒体活性,体完整性,活力和氧化应激标志物来评估.
- 参数包括类似于亡的变化,脂质过氧化,DNA碎片化和活性氧物种 (ROS) 度.
主要成果:
- 与其他组相比,10μM CoQ10 组 (Q10) 显示出明显更高的总运动性,渐进运动性,平均路径速度,膜完整性,线粒体活性,体完整性和活力.
- 补充10μM的CoQ10显著降低了类似apoptotic的变化,脂质过氧化,DNA碎片化和ROS度.
- CoQ10表现出剂量依赖的保护作用,10μM是最有效的度.
结论:
- 辅酶Q10作为一种强大的抗氧化剂,显著提高了冷保存的雄鹿精液的质量和生育潜力.
- 豆 lecithin 扩展剂中的 10 μM CoQ10 度是保护雄鹿精子免受冷损伤的最佳选择.
- 补充辅酶Q10是一种有前途的策略,可以提高小型反动物精液冷保存用于生殖目的的疗效.
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