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基于ICP-MS的检测方法与Au NP和Ag NP标签相结合,用于细菌病诊断

Xiaoting Li¹, Xin Li¹, Jiayi Chen¹

¹Department of Food and Drug, ShenZhen Polytechnic University, Liuxian Avenue, Nanshan District, Shenzhen, Guangdong, 518055, China.

Analytical biochemistry

|May 9, 2024

概括

这项研究引入了一种快速检测血中的沙门氏菌和大肠杆菌 (大肠杆菌O157:H7) 的方法,使用ICP-MS和纳米粒子. 这种方法有助于早期诊断细菌病,改善患者的治疗结果.

科学领域:

分析化学分析化学
生物技术是生物技术.
传染病诊断传染病诊断传染病诊断

背景情况:

细菌病症是一个重大的健康挑战,其发病率不断增加,死亡率高.
早期和准确的诊断对于有效的治疗和改善患者存活率至关重要.
目前的诊断方法可能缺乏及时干预所需的速度和特异性.

研究的目的:

开发一种新的,快速的,同时检测人的血液中的沙门氏菌和大肠杆菌O157:H7的新方法.
使用感应合等离子体质谱法 (ICP-MS) 结合纳米粒子标记,以提高细菌检测.
为早期诊断细菌血症建立一种敏感和特定的测定方法.

主要方法:

使用ICP-MS.同时检测沙门氏菌和大肠杆菌O157:H7.
使用磁珠捕获细菌和特定抗体功能化的金纳米粒子 (Au NP) 和银纳米粒子 (Ag NP) 进行信号放大.
优化实验条件以达到高灵敏度和特异性.

主要成果:

达到了164 CFUmL-1的沙门氏菌和220 CFUmL-1的大肠杆菌O157:H7.7.的检测极限.
确定的线性范围为沙门氏菌的40080,000 CFU mL-1和大肠杆菌O157:H7.1的40060,000 CFU mL-1.
在3.5小时内在人体全血中同时检测出两种病原体.

结论:

开发的基于ICP-MS的方法与纳米粒子标签提供了一个快速和同时的方法来检测关键的细菌病原体.
这种技术显示出在改善临床环境中细菌病诊断的速度和准确性的巨大潜力.
该方法在检测沙门氏菌和大肠杆菌O157:H7方面的效率有助于推进传染病诊断.

关键词:

细菌病的诊断细菌病的诊断基本元素标签基本元素标签在ICP-MS中,使用的是ICP-MS.

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