抗生素诱导的微生物群枯竭促进了Campylobacter jejuni在小鼠中的肠道殖民
Haohao Chen1, Yanfang Zhang2, Yi Pan2
1Medical Molecular Biology Laboratory, School of Medicine, Jinhua Polytechnic, No. 1188 Wuzhou Street, Wucheng District, Jinhua, Zhejiang Province, P.R. China. 20101044@jhc.edu.cn.
BMC microbiology
|May 9, 2024
概括
抗生素治疗削弱了小鼠的肠道微生物群,使长期的Campylobacter jejuni殖民成为可能. 这种方法建立了一个研究C. jejuni感染及其对肠道环境的影响的模型.
科学领域:
- 微生物学 微生物学
- 胃肠病学 胃肠病学
- 动物模型 动物模型
背景情况:
- 坎比洛巴克特 (Campylobacter jejuni) 是全球细菌性胃肠炎的主要原因之一.
- 建立可靠的C. jejuni殖民的动物模型对于研究至关重要.
- 抗生素诱导的微生物群枯竭是一种促进病原体殖民的潜在策略.
研究的目的:
- 开发一种方法来诱导C. jejuni在C57BL/6小鼠的肠道殖民.
- 调查抗生素诱导的微生物群枯竭对C. jejuni殖民化的影响.
- 为了描述由此导致的肠道微生物群组成的变化.
主要方法:
- 在C57BL/6小鼠中,使用Cefoperazone sodium和sulbactam sodium进行了微生物群枯竭.
- 小鼠通过口腔输液接种了C. jejuni,并给予了强剂量.
- 使用TaqMan qPCR监测便中的C. jejuni水平.
- 通过免疫光学和组织学来评估肠道殖民.
- 用16S rDNA测序分析了肠道微生物组成.
主要成果:
- 在实验组的便和肠道光线中成功检测到C. jejuni14天.
- 肠道组织显示了最小的炎症和完整的粘膜.
- 结肠微生物群的显著变化被观察到,其中增加了Bacteroidetes和减少了Firmicutes.
- 实验组和对照组之间的微生物群组成有显著差异.
结论:
- 和硫巴克坦有效地消耗了肠道微生物群,促进了C. jejuni的殖民.
- 这种抗生素诱导的模型允许在小鼠肠道中持续存在C. jejuni.
- 这项研究为研究C. jejuni病变和宿主微生物相互作用提供了宝贵的工具.
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