一个有效和全面的优化策略,用于制备由宏树脂列染色学致富于石基胺酸的银杏花叶提取物
Sijie Zhang1,2, Xingchu Gong1, Haibin Qu1
1Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, China.
Phytochemical analysis : PCA
|May 10, 2024
概括
这项研究优化了使用宏树脂染色学优化银杏叶提取物 (EGBL) 制备,以丰富黄类甘油酸 (FG),三 (TT) 和石基胺酸 (SA). 开发的方法可以有效地提取这些化合物,从而使G. biloba叶 (GBL) 的高价值利用.
科学领域:
- 植物化学 植物化学
- 自然产品化学 自然产品化学
- 染色学分离科学 染色学分离科学
背景情况:
- 传统的银杏花叶提取物 (EGBL) 制备方法主要侧重于黄类糖化物 (FG) 和类三酸盐 (TT).
- 这种关注导致了G. biloba叶 (GBL) 中有价值化合物的不足利用.
- 存在对最大限度地从GBL中恢复多个活性成分的方法的需求.
研究的目的:
- 开发一个全面的优化策略,以使用宏树脂列色谱来准备EGBL.
- 为了同时从GBL中丰富类糖化物 (FG),类三 (TT) 和石基胺酸 (SA).
- 建立一个高效和可扩展的方法,用于高价值的G. biloba叶子的利用.
主要方法:
- 通过静态吸附/脱附实验对宏树脂进行选.
- 优化溶液pH的吸附和脱吸.
- 使用最终选设计 (DSD) 系统地调查八个过程参数.
- 扩大规模的验证实验以评估工业可行性.
主要成果:
- 在pH值2.0下,XDA1树脂被确定为最佳吸附剂.
- 使用DSD确定了优化的参数范围,证实了其用于EGBL准备的可靠性.
- 扩大规模的实验结果显示,EGBL含有26.54%的FG,8.96%的TT和10.70%的SA,符合标准EGB761的SA含量.
结论:
- 开发了一种有效和方便的方法来制备SA丰富的EGBL.
- 该战略有助于高价值地利用G. biloba的叶子.
- 这种方法增强了从GBL中恢复多个关键化合物的能力.
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