在临床样本中使用长读单分子实时测序进行HPV基因型鉴定
Christophe Pasquier1, Stéphanie Raymond1, Romain Carcenac1
1Department of Virology, Toulouse University Hospital, Toulousel, France.
Journal of medical virology
|May 10, 2024
概括
使用PacBio SMRT测序,可以使用E6/E7区域进行准确的人类乳头瘤病毒 (HPV) 基因型鉴定. 这种方法有效检测多种HPV感染,也是用于宫癌查的宝贵工具.
科学领域:
- 分子生物学分子生物学
- 病毒学 病毒学
- 遗传学 是一个遗传学.
背景情况:
- 人类乳头瘤病毒 (HPV) 基因型鉴定对于宫癌查至关重要,通常与高风险 (HR) HPV检测相结合.
- 现有的方法,如L1测序和APTIMA HPV基因定型,在全面的HPV分析中存在局限性.
研究的目的:
- 开发和评估一种使用PacBio单分子实时 (SMRT) 测序E6/E7区域的新型HPV基因型定型方法.
- 评估这种新方法的性能与既有技术 (MY09-11 L1测序和APTIMA HPV基因定型) 相比.
主要方法:
- 使用PacBio SMRT测序分析了HPV的E6/E7区域中的大约800bp序列.
- 将PacBio E6/E7 SMRT基因型鉴定的性能与MY09-11 L1测序和APTIMA HPV基因型鉴定试验进行了比较.
主要成果:
- PacBio E6/E7 SMRT测序显示与MY09-11 L1测序100%一致,与APTIMA HPV基因型鉴定有90.8%的一致性.
- 正如预期的那样,PacBio E6/E7 SMRT的灵敏度与L1测序相比较,低于HR-HPV测试.
- 帕克比奥SMRT测序提供了无偏见的基因型定型,能够检测多种HPV感染和基因型内变异 (哈普型).
结论:
- 在临床样本中,PacBio E6/E7 SMRT测序是高风险 (HR) 和低风险 (LR) HPV基因定型的强大而准确的方法.
- 这种技术在宫癌查协议中,在阳性HPV测试后作为后续基因定型检测特别有用.
- 检测多种感染和单元型的能力使PacBio SMRT测序在HPV诊断中取得了宝贵的进步.
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